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培养基

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培养基及其组成


0001. 醋酸菌培养基

葡萄糖    100.0 g

酵母提取物    10.0  g

CaCO3    20.0  g

琼脂   15.0  g

蒸馏水   1.0  L

pH    6.8


0002. 营养肉汁琼脂

蛋白胨    10.0 g

牛肉提取物    3.0 g

NaCl  5.0 g

琼脂    15.0 g

蒸馏水     1.0 L

pH   7.0


0003. 固氮培养基

KH2PO4   0.2 g

K2HPO4  0.8 g

MgSO4·7H2O  0.2 g

CaSO4·2H2O   0.1 g

FeCl3     微量 g

Na2MoO4·2H2O   微量 g


0006. MRS培养基

酪蛋白胨  10.0 g

牛肉提取物   10.0 g

酵母提取物    5.0 g

葡萄糖   5.0 g

乙酸钠   5.0 g

柠檬酸二胺   2.0 g

Tween 80   1.0 g

K2HPO4   2.0 g

MgSO4·7H2O  0.2 g

MnSO4·H2O 0.05 g

CaCO3  20.0 g

琼脂  13.0 g

蒸馏水   1.0 L

pH   6.8


0007. PYG培养基

蛋白胨   10.0 g

酵母提取物  5.0 g

葡萄糖   1.0 g

琼脂   15.0 g

蒸馏水  1.0 L 

酵母提取物  0.5 g

甘露醇   20.0 g

琼脂   15.0 g

蒸馏水  1.0 L

pH   7.2


0004. 玉米粉培养基

玉米粉   5.0 g

蛋白胨  0.1 g

葡萄糖    1.0 g

琼脂   13.0 g

蒸馏水   1.0 L

自然pH


0005. 乳酸菌培养基

酵母提取物   7.5 g

蛋白胨  7.5 g

葡萄糖   10.0 g

KH2PO4  2.0  g

番茄汁   100.0 ml

Tween 80   0.5 ml

蒸馏水   900  ml

pH    7.0

pH   6.8-7.0


0008. 甘油琼脂

蛋白胨     5.0 g

牛肉提取物    3.0 g

甘油   20.0 g

琼脂   15.0 g

蒸馏水   1.0 L

pH   7.0-7.2


0009. 根瘤菌培养基

酵母提取物      1.0 g

甘露醇   10.0 g

土壤提取液   200 ml

琼脂    15.0 g

蒸馏水    800 ml

pH   7.2

土壤提取液:土壤50g, 加水200ml, 121℃蒸煮1小时, 过滤后加水补足到200ml


0010. 甘露醇琼脂

酵母提取物    5.0 g

蛋白胨   3.0 g

甘露醇  25.0 g

琼脂   15.0 g

蒸馏水   1.0 L

pH   7.0


0011. 葡萄糖、天门冬素琼脂

葡萄糖   10.0 g

天门冬素   0.5 g

K2HPO4   0.5 g

琼脂   15.0 g

蒸馏水   1.0 L

pH    7.2-7.4


0012. 高氏合成一号琼脂

可溶性淀粉  20.0 g

FeSO4·4H2O  0.01 g

K2HPO4   1.0 g

琼脂  15.0 g

蒸馏水   1.0 L

pH   6.0-6.5


0017. 综合PDA琼脂

马铃薯提取液   1.0 L

葡萄糖  20.0 g

KH2PO4   3.0 g

MgSO4·7H2O    1.5 g

维生素B1    微量 g

琼脂  15.0 g

KNO3   1.0 g

K2HPO4    0.5 g

MgSO4·7H2O   0.5 g

NaCl   0.5 g

FeSO4  0.01 g

琼脂  15.0 g

蒸馏水     1.0 L

pH   7.2-7.4


0013. 麦芽汁琼脂

12 Brix. 麦芽汁   1.0 L

琼脂  15.0 g

自然pH


0014. 马铃薯、葡萄糖、琼脂(PDA

取去皮的马铃薯200g,切成小块,加水1000ml煮沸30分钟,滤去马铃薯块,将滤液补足至1000ml,加葡萄糖20g,琼脂15g,溶化后分装,115℃灭菌20分钟。


0015. Czapeks 琼脂

蔗糖       30.0 g

NaNO3   3.0 g

MgSO4·7H2O   0.5 g

KCl   0.5 g

FeSO4·4H2O  0.01 g

K2HPO4   1.0 g

琼脂   15.0 g

蒸馏水   1.0 L

pH   6.0-6.5


0016. 浓糖查氏琼脂

蔗糖    200.0 g

NaNO3   3.0 g

MgSO4·7H2O   0.5 g

KCl   0.5 g

pH   6.0


0018. 玉米粉培养基

玉米粉   200 g

蒸馏水   1.0 L

自然pH


0019. 滤纸培养基

(NH4)2SO4   1.0 g

KH2PO4   1.0 g

MgSO4·7H2O   0.7 g

NaCl   0.5 g

蒸馏水   1.0 L

pH   7.0

每个试管放入一条滤纸(6×1厘米)


0020. 黄豆饼粉培养基

10%黄豆饼粉提取液   1.0 L

葡萄糖  10.0 g

NaCl   2.5 g

琼脂   15.0 g

自然pH


0021. 木屑麸皮培养基

木屑(阔叶树)   75 g

麸皮   25 g

   适量

自然pH


0022. 松木条(屑)培养基

松木屑  75.0 g

米糠  23.0 g

蔗糖   2.0 g

  适量

自然pH


0023. 稻草、马粪培养基

干马粪(或牛粪)  65%

干稻草   35%

尿素  0.5%

硫酸铵  0.3%

过磷酸钙  0.7%

石膏粉  1%

水适量进行堆积发酵


0024. 稻草米糠培养基

干稻草  75-80%

米糠  20-25%

选无霉稻草,切成长约3cm的小段,用清水浸泡12小时。使充分吸水。然后倾去水,加米糠和水,水量以用手捏时指间有水珠渗出为宜。


0025. 米饭培养基

取大米10g,入50ml锥形瓶中,加水20-30ml121℃灭菌30分钟即成,或取大米2g,入15×150mm试管中,加水6ml,于121℃磅灭菌30分钟。


0026. 滤纸条培养基2

(NH4)2SO4   1.0 g

KH2PO4    1.0 g

MgSO4·7H2O   0.5 g

酵母提取物   0.1 g

蒸馏水   1.0 L

自然pH

每个试管放入一条滤纸(7×1厘米)


0027. YM Agar (1)

酵母提取物  10.0 g

麦芽提取物  10.0 g

葡萄糖   4.0 g

琼脂  15.0 g

蒸馏水   1.0 L

pH   7.0

可溶性淀粉  10.0 g

NaCl   5.0 g

CaCO3   3.0 g

琼脂  15.0 g

蒸馏水   1.0 L

pH   7.0


0030. 麸皮培养基1

麸皮   36 g

(NH4)2HPO4   3.0 g

K2HPO4   0.2 g

MgSO4·7H2O   0.1 g

琼脂  15.0 g

蒸馏水   1.0 L

pH   7.0


0031. 酵母菌生孢子培养基

KH2PO4    0.12 g

K2HPO4    0.2 g

Sodium acetate   5.0 g

Glucose  0.62 g

NaCl  0.62 g

Peptone   2.5 g

Biotin   微量

Agar   15 g

Trace element solution  10 ml

蒸馏水   1.0 L

自然pH

Trace element solution:

MgSO4·7H2O   0.4 g

NaCl    0.4 g

CuSO4·5H2O   0.002 g

MnSO4·4H2O   0.2 g

FeSO4·4H2O    0.2 g

蒸馏水   100.0 ml


0028. YP琼脂

酵母提取物   1.0 g

牛肉提取物   1.0 g

多胨   2.0 g

葡萄糖  10.0 g

琼脂  15.0 g

蒸馏水   1.0 L

pH   7.0

0029. GYS琼脂

葡萄糖  10.0 g

0032. BPY琼脂

牛肉提取物   5.0 g

蛋白胨      10.0 g

酵母提取物   5.0 g

葡萄糖  5.0 g

NaCl   5.0 g

琼脂   15.0 g

蒸馏水   1.0 L

pH   7.0

0033. LB培养基


酵母提取物  10.0 g

酵母提取物   5.0 g


蛋白胨  10.0 g

NaCl   10.0 g

琼脂  15.0 g

蒸馏水   1.0 L

pH   7.0

0034. Penessay 琼脂

牛肉提取物   1.5 g

酵母提取物   1.5 g

胰蛋白胨   5.0 g

NaCl  0.01 g

MnSO4  0.01 g

FeSO4  0.01 g

CaCO3    5.0 g

琼脂   15.0

蒸馏水   1.0 L

pH   7.0

0038. ISP-2培养基

酵母膏  4g


葡萄糖   1.0 g

NaCl   3.5 g

K2HPO4   4.8 g

KH2PO4  1.32 g

琼脂  15.0 g

蒸馏水   1.0 L

pH   7.2

0035. 血琼脂培养基-1

蛋白胨   10.0 g

葡萄糖  2 g

NaCl   5 g

NaH2PO4    2.5 g

柠檬酸钠  5 g

脑浸汁 800ml

猪心浸汁 200ml

羊血  10%

pH  7.2

0036. 纤维细菌合成培养基

NaCl   6.0 g

MgSO4·7H2O   0.1 g

KH2PO4   0.5 g

CaCl   0.1 g

K2HPO4   2.0 g

(NH4)2SO4   2.0 g

酵母提取物   1.0 g

滤纸条    1

蒸馏水   1.0 L

pH   7.0-7.5

0037. 巴氏梭菌合成培养基

葡萄糖   10.0 g

酵母提取物   0.1 g

蛋白胨   0.1 g

KH2PO4   0.5 g

K2HPO4   0.5 g

MgSO4·7H2O   0.2 g

葡萄糖  4g

琼脂  17g

麦芽膏  10g

蒸馏水  1000ml

pH   7.3

0039. 燕麦粉琼脂-1 (ISP-3)

燕麦粉  20.0 g

微量盐溶液  1.0 ml

琼脂  15.0 g

蒸馏水   1.0 L

pH  7.2

微量盐溶液:

FeSO4·7H2O  0.1 g

MnCl2·4H2O   0.1 g

ZnSO4·7H2O   0.1 g

蒸馏水   100.0 ml

0040. 苹果酸钙琼脂

苹果酸钙   10.0 g

甘油  10.0 g

K2HPO4  0.05 g

NH4Cl   0.5 g

琼脂  15.0 g

蒸馏水   1.0 L

pH  7.2

0041. 氧化亚铁硫杆菌培养基

(NH4)2SO4  0.15 g

KH2PO4  0.05 g

KCl  0.05 g

MgSO4·7H2O   0.5 g

Ca(NO3)2·4H2O  0.01 g

FeSO4·7H2O   50.0 g

Distilled water   1.0 L

pH   2.0

0042. 氧化硫硫杆菌培养基

(NH4)2SO4   0.3 g

KH2PO4   3-4 g

CaCl2 (anhydrous)  0.25 g

MgSO4·7H2O   0.5 g

FeSO4·7H2O   0.001 g

Distilled water   1.0 L

Distilled water    1.0 L

pH  7.0

0049.

Peptone    10.0 g

NaCl   5.0 g


pH   3.5-4

0043. Plasma Substitute Me

蔗糖   130.0 g

Yeast extract   5.0 g

Distilled water   1.0 L

pH   7.0


蛋白胨   2.0 g

KH2PO4   0.3 g

Na2HPO4   1.4 g

琼脂  15.0 g

蒸馏水   1.0 L

pH  7.0-7.2

0044. 脱脂牛奶培养基

脱脂奶粉   100.0 g

蒸馏水   1.0 L

自然pH

0045. 豌豆琼脂

葡萄糖  10.0 g

蛋白胨   5.0 g

豌豆提取液(以NH2计每100ml12-14mg 1.0 L

琼脂  15.0 g

pH  7.2-7.5

0046. GY琼脂

葡萄糖   10.0 g

酵母提取物  10.0 g

琼脂   15.0 g

蒸馏水   1.0 L

pH  7.2

0047.

酪蛋白胨  10.0 g

葡萄糖   1.0 g

酵母提取物  5.0 g

NaCl  5.0 g

琼脂  15.0 g

蒸馏水   1.0 L

pH  7.2

0048.

Beef extract   3.0 g

Peptone   5.0 g

NaCl   5.0 g

Glucose  10.0 g

0050.

Peptone  39.0 g

Beef extract   5.0 g

Glucose   1.0 g

NaCl   5.0 g

Distilled water   1.0 L

pH  7.2

[note] Used for solid medium, agar 18-20g for low layer and 8-10g for uper layer

0051. 麦芽汁琼脂-2

15 Brix.麦芽汁   1.0 L

琼脂  15.0 g

自然pH

0052. 厌氧肉肝培养基

Beef    250.0 g

Liver(ox or sheep)   250.0 g

Peptone  10.0 g

Glucose   2.0 g

NaCl  5.0 ml

Liver minces   100.0 g

Distilled water   1.0 L

pH  7.8-8.0

PREPARATION:

(1)Immerse 250g of minced beef and 250g of liver block into the water for 20-24 hours.

(2)Boil 30-60 min then filtrate by cloth, then add water to make up the filtrate to 1000ml.

0053.

Ox heart infusion    1000 ml

Tryptone   10.0 g

Proteose peptone   2.5 g

Peptone   2.5 g

Glucose   5.0 g

Agar   1.0 g

Na2HPO4 (anhyd.)   4.2 g

KH2PO4    0.54 g

Dry ox heart pieces  suitable amount

PREPARATION:

(1) Mix and dissolve the ingredants in to ox heart infusion. Adjust pH to 7.6-7.8.Boil the broth for 10 min..Fil-trate by paper

(2) Distribute the broth into tubes (each contain-ing liver pieces about 1/2 v/v of the broth)

(3) Distribute into tubes (each tube containing

1/2 beef pieces of the volume of the broth);

covered with paraffin oil; autoclave at 8 lb for

30 minutes

0056. 马丁氏培养基

Beef infusion   500.0 ml

Pig stomach digested broth  500.0 ml

Agar    15.0 g


(3) Covered by paraffin oil,then autoclove at 10 lb for 30 minutes

0054.

Beef   200.0 g

pH  7.0-7.6

[note]

(1) Adjust pH 7.6 and add 5-10% fresh sheep

blood, when used for cultivation of B.


Liver (ox or sheep)   50.0 g

Peptone   10.0 g

Dextrin   10.0 g

HCl   10.0 ml

Pepsin(1:3000)    3.0 g

Distilled water    1.0 L

PREPARATION:

(1) Put the minced beef and liver into the water, add HCl and pepsin in it,then stir.

(2) Digest the meat at 53-55 C for 22-24 hours; stir them every hour at the first 10 hours.

(3) Heat the supernatant to 80 C;add peptone; adjust the pH to 7.6-7.8; boil for 10 min.

(4) Add dextrin after filtration; distri-bute the broth in tubes; covered by paraffin oil, autoclave at 6lb for 20 min.

[note] Add the rusty iron filings about 1/10 volume of the broth, when used for cultivation of Clostridium novyi

0055.

erysipelatos-suis

(2) Adjust pH 7.0 and add 5-10% horse serum

used for cultivation of Brucella

0057. 支原体培养基

Hanks 1% casein hydrolyzed liquid 400.0 ml

Serum(horse)   160.0 ml

Ox heart broth   240.0 ml

Yeast extract liquid   16.0 ml

1% Thallium acetate  8.0 ml

Penicillin  200000.0 U

PREPARATION:

(1) Ox heart broth: Immerse 1000g of minced

heart into 1600ml water at 80 for 10 min.;

Chill it to 45 and adjust pH8.2-8.5 with

Na2CO3, then add pancreatic juice 33ml and

chloroform 5-10ml; Digest it at 45 for 3

hours, and stir it half hour interval and adjust

pH at same time; Adjust pH to 4-4.5 with HCl,

boil for 30 min, filtrate with cloth; Adjust the

filtrate pH to 7.8-8.0, boil for 30 min, and


Beef   500.0 g

Peptone   10.0 g

NaCl   5.0 g

Glucose  10.0 g

Distilled water   1.0 L

PREPARATION:

(1) immerse the thin and minced beef in cold water and stay for overnight; boil for 40 min.; filtrate by cloth.

filtration by paper; Distribute the broth in to

flask, autoclave at 8 bl for 20 min.

(2)Yeast extract liquid: Put braking yeast 500g

into 1000ml of water, full mix and adjust ph to

5.0; Heat to 80 for 30 min; After filtration

and distribution, autoclave at 8 bl for 30 min.

0058. 马丁氏肉汤

Beef infusion Broth   500.0 ml

Pig Stomach Digested Broth   500.0 ml


(2) Add the peptone and NaCl in the filtrate;

adjust pH to 7.8-8.0; boil for 10 min.;

add glucose after filtration.

NaCl   2.5 g

pH  7.4-7.6

0059. 4%甘油培养基

Beef extract broth   1.0 L

Peptone    10.0 g

Yeast extract   1.0 g

Iron citrate(1%)  1.0 ml


NaCl   5.0 g

Glycerol  40.0 g

Boric acid (1%)  1.0 ml

Agar    15.0 g


Agar  15.0 g

Distilled water   1.0 L


pH  7.0-7.2

0060. 沙博若琼脂

Glucose    40.0 g

Peptone  10.0 g

Agar  15.0 g

Distilled water   1.0 L

pH   5.6

0061.

Fresh milk    150.0 ml

Potato starch   6.0 g

Peptone   1.0 g

Potato (whole)  1

Egg    5

Glycerol   12.0 ml

1% Malachite green  3.0 ml

PREPARATION:

(1) Dice one potato, which striped skin; add milk, peptone and potato starch in a beaker; heat in boiling water bath for 10 min after mixed; pound the potato to paste.

(2) Further heat for 1 hour; add 4 egg liquid, 1 egg yolk, the glycerol and malachite green; filtrate through gauze after full mixed.

(3) Distribute into tubes; slant the tubes in a sero-coagulator and sterilize intermittently.

0062. 肝汤琼脂

Liver infusion (1:2)   1.0 L

Peptone     10.0 g

NaCl   5.0 g

Agar  15.0 g

pH   6.8-7.0

0063. 根瘤菌琼脂-1

Sucrose (or Mannitol)   10.0 g

MgSO4·7H2O   0.2 g

K2HPO4    0.5 g

CaSO4   0.2 g

NaCl    0.1 g

NaMoO4(1%)   1.0 ml

MnSO4(1% )  1.0 ml

pH   6.8-7.0

0064. 根瘤菌琼脂-2

甘油  10ml

NaCl  0.1g

酵母片  4

K2HPO4·3H2O   0.5 g

CaCO3­   3g

MgSO4·7H2O   0.2 g

琼脂  15-20g

 1000ml

0065. 固氮菌琼脂

Sucrose or Mannitol    10.0 g

CaCO3   1.0 g

K2HPO4·3 H2O   0.5 g

MgSO4·7H2O   0.2 g

NaCl   0.2 g

Agar    15-20 g

Distilled water   1000.0 ml

pH   7.0-7.2

0066. 马铃薯蔗糖琼脂

Potato infusion   500.0 ml

Sucrose  20.0 g

Agar  20.0 g

Distilled water   500.0 ml

自然pH

[note] Stripped and diced potato 1800g, wrap

them with cloth and put the pack into 4500ml

of water; boil for 10 minutes, then discard the

potato dices; autoclave at 15 lb for 20 min.,

and store the infusion in refrigerator for

usage.

0067. 钾细菌琼脂

Sucrose    10.0 g

K2HPO4   0.5 g

Yeast extract   0.4 g

MgSO4·7H2O   0.2 g

MgCl2   0.2 g

Agar  15.0 g

Distilled water   1.0 L

pH   7.0-7.2

0068. 金氏B琼脂

Peptone  20.0 g

K2HPO4   1.5 g

MgSO4·7H2O   1.5 g

Glycerol    10.0 g

NaCl   0.2 g

CaSO4·2H2O   0.2 g

CaCO3   5.0 g

Agar  15.0 g

Distilled water   1.0 L

0074. 淀粉铵琼脂


Agar  20.0 g

Distilled water   1.0 L

Adjust pH 7.2 by use of KOH

0069. 玉米粉黄豆饼粉培养基

Corn meal   20.0 g

Soybean meal  10.0 g

Glucose    20.0 g

NaCl   5.0 g

(NH4)2SO4   3.0 g

Agar  15.0 g

Water   1.0 L

pH nature

[note] Boil corn and soybean meal in water separately; filtrate through gauze; use the filtrate.

0070. 淀粉琼脂

Soluble starch  10.0 g

NaNO3   1.0 g

MgCO3   1.0 g

K2HPO4   0.3 g

NaCl   0.5 g

Agar    20.0 g

Distilled water   1.0 L

0071. 甘油琼脂-2

Beef infusion   350.0 ml

Glycerol  60.0 g

Peptone  10.0 g

Agar  15.0 g

Make to 1000ml with nutrient broth; adjust pH to 7.3; autoclave at 15 lb for 15 minutes

0072. 麦芽膏琼脂

Malt extract  20.0 g

Agar    20.0 g

Top water   1.0 L

0073. 阿氏无氮培养基

Mannitol  10.0 g

K2HPO4   0.2 g

MgSO4·7H2O   0.2 g

(NH4)2SO4   2 g

K2HPO4    1 g

NaCl   1 g

CaCO3   3 g

MgSO4   1 g

淀粉  10 g

琼脂  20 g

蒸馏水  1000ml

pH    7.2-7.4

0075. 银耳芽孢培养基

Potato (stripped and diced)   250.0 g

Sucrose  20.0 g

KH2PO4   1.0 g

(NH4)2SO4   2.0 g

Agar  15.0 g

Distilled water     1.0 L

pH   7.0

[note] Boil the potato in water for 30 minutes;

filtrate through gauze; add the ingredients to

the filtrate

0076. 4 Be麦芽汁琼脂

4 Brix. 麦芽汁   1.0 L

琼脂   15.0 g

自然pH

0077. 5 Be麦芽汁琼脂

5 Brix. 麦芽汁   1.0 L

琼脂  15.0 g

自然pH

0078.

0079. 6-8 Be麦芽汁琼脂

8 Brix. 麦芽汁   1.0 L

琼脂  15.0 g

自然pH

0080. 5 Be麦芽汁琼脂

5 Brix. 麦芽汁   1.0 L

琼脂  15.0 g

pH   6.0

0081. 单宁麦芽汁琼脂

5 Brix 麦芽汁    1.0 L

单宁  10.0 g

琼脂  15.0 g

自然pH

0082. 土茯苓麦芽汁琼脂

FeSO4·4H2O   0.1 g

Agar  15.0 g

Distilled water   1.0 L

0090. 马铃薯培养基-1

Stripped and minced potato   200.0 g


5 Brix 麦芽汁   40.0 ml

土茯苓汁   60.0 ml

琼脂   1.5 g

自然pH

0083. 5 Be麦芽汁琼脂-2

5 Brix. Wort   1.0 L

Yeast extract   5.0 g

CaCO3   6.0 g

琼脂  15.0 g

pH nature

0084. 5 Be米曲汁琼脂

5 Brix. Koji extract   1.0 L

琼脂  15.0 g

pH nature

0085. 6-7 Be米曲汁琼脂

6-7 Brix. Koji extract   1.0 L

琼脂    15.0 g

pH nature

0086. 饴糖培养基

8% Malt sugar solution   1.0 L

Peptone   5.0 g

Agar    15.0 g

pH nature

0087. 6-7 Be 饴糖培养基

6-7 Brix Malt sugar solution   1.0 L

Peptone   5.0 g

Agar    15.0 g

pH nature

0088. 10-12%糖蜜培养基

Molasses   100.0 g

NH4NO3   5.0 g

Agar    15.0 g

Distilled water   1.0 L

0089. 查氏琼脂-2

Glucose  50.0 g

NaNO3   2.0 g

MgSO4·7H2O   0.5 g

K2HPO4   1.0 g

KCl   0.5 g

Distilled water   1.0 L

Agar     15.0 g

pH nature

[note] Boil potato in water for 1hr. filtrate

through gauze; add water to the filtrate to

make up 1000ml

0091. 马铃薯培养基-2

Potato infusion   20%

Glucose   1%

Agar     2%

pH   6.8

0092. 马铃薯滤纸培养基

Potato infusion   1000 ml

Glucose   10 g

Agar   25 g

pH   6.7-6.9

Filter paper strip

[note] Sterilize the filter paper strip separately,

then put the strip on the slant

0093. 滤纸琼脂

Filter paper   1.5%

(NH4)2SO4  0.05%

MgSO4·7H2O  0.01%

K2HPO4    0.025%

Agar     2%

0094. 麸皮马铃薯琼脂

Wheat-Potato infusion   100 ml

Sucrose   2%

Agar   2%

pH  6

[note] Boil 5g of wheat bran and 20g of diced

potato in water for5 min. filtrate, add water to

the filtrate to make up 100ml

0095.

Beef extract   0.5%

Yeast extract   0.5%

Peptone   1%

Glucose   1%

Lactose   0.5%

NaCl   0.5%

Agar    2%

pH   6.8

0101. 牛肉膏淀粉培养基

Beef extract  20.0 g

Peptone    10.0 g


0096. 西红柿汁琼脂

Peptone   10 g

Glucose   10 g

Beef extract   10 g

Yeast extract   5 g

Tween 80  0.5 ml

Tomato juice   200 ml

Agar   20 g

Top water   800 ml

0097. BY琼脂

Beef extract   0.5%

Yeast extract   0.5%

Peptone   0.5%

NaCl  0.25%

Agar    2%

pH   7.0

0098. 牛肉膏果胶培养基-1

Beef extract    1.0%

Peptone   1.0%

Pectin   1.0%

Glucose   0.5%

K2HPO4   0.1%

NaCl   0.3%

Agar   2%

pH   7.0

0099. 牛肉膏果胶培养基-2

Beef extract   1.0%

Peptone   0.5%

Pectin   0.5%

K2HPO4   0.1%

NaCl   0.5%

Agar   2%

pH   7.5

0100. 葡萄糖营养琼脂-2

Beef extract   10.0 g

Peptone  10.0 g

Glucose  10.0 g

NaCl   5.0 g

Agar  15.0 g

Distilled water   1.0 L

pH   7.0

Starch  20.0 g

Agar    15.0 g

Distilled water   1.0 L

pH   7.2

0102. 牛肉膏石蜡培养基

Beef extract   10.0 g

Peptone  10.0 g

NaCl   5.0 g

Liquid paraffin Small volume

Agar  15.0 g

Distilled water   1.0 L

pH   7.0

0103. BY培养基

Beef infusion   1.0 L

Peptone  10.0 g

NaCl   5.0 g

Yeast extract   5.0 g

Agar  15.0 g

pH   7.0

0104. 心浸液培养基

Heart infusion (Difco 0038)  12.5 g

Nutrant broth (Difco 0003)   5.4 g

Yeast extract   2.5 g

Agar    15.0 g

Distilled water   1.0 L

0105. TY培养基-1

Tryptone  20.0 g

Yeast extract   5.0 g

FeCl3·6H2O   7.0 mg

MnCl3·4H2O   1.0 mg

MgSO4·7H2O   15.0 mg

Agar   15.0 g

Distilled water   1.0 L

[note] Autoclave at 15lb for 15 min.; then

adjust pH to 7.3 with sterilized 10% KOH;

add sterilized fructose 0.5% as carbon source.

0106. 蜂蜜培养基

Honey  60.0 g

Peptone  10.0 g

Agar  15.0 g

Distilled water   1.0 L


pH   6.4-6.6

0111. 3.5%NaCl营养琼脂


0107. 柠檬酸铁铵培养基

K2HPO4   0.5 g

MgSO4·7H2O   0.5 g

Iron ammonium citrate   0.5 g

Glycerol  20.0 g

Citric acid   2.0 g

L-glutamic acid   4.0 g

Agar  15.0 g

Distilled water   1.0 L

pH   7.4

0108.

Glucose  10.0 g

(NH4)H2PO4   1.0 g

MgSO4·7H2O   0.2 g

KCl    0.2 g

Beef extract   3.0 g

Yeast extract   5.0 g

Peptone   5.0 g

Agar  15.0 g

Trace element  1.0 ml

Distilled water   1.0 L

pH   7.2

Trace element solution:

Sodium tartarate   88.0 mg

Ammonium molybdenate   37.0 mg

FeCl2   97.0 mg

ZnSO4   800.0 mg

MnCl2   72.0 mg

distilled water   1.0 L

0109. 血琼脂-2

Beef infusion (1:3)   1.0 L

Peptone  10.0 g

NaCl   5.0 g

Agar  15.0 g

Defibrillated sheep blood   50.0 ml

pH   7.2-7.4

0110. 巧克力色血琼脂

Beef infusion (1:3)   1.0 L

Peptone  10.0 g

NaCl     5.0 g

Agar   15.0 g

Add defibrillated sheep blood at 80-90 C

pH    7.2-7.4

Beef infusion (1:3)   1.0 L

Peptone  10.0 g

NaCl  35.0 g

Agar   15.0 g

pH   7.2-7.4

0112. 肝琼脂

Pig liver infusion   500.0 ml

Distilled water   500.0 ml

Peptone  10.0 g

NaCl     5.0 g

Agar  15.0 g

pH   6.8-7.0

0113. 链霉素琼脂

Beef infusion (1:3)   1.0 L

Peptone  10.0 g

NaCl   5.0 g

Agar    15.0 g

pH     7.2-7.4

0114. 柯托夫琼脂

Peptone   0.8 g

NaCl   1.4 g

NaHCO3   20.0 mg

KCL   40.0 mg

CaCl2   40.0 mg

KH2PO4   0.24 g

Na2HPO4   0.88 g

Distilled water  1.0 L

pH   7.0-7.3

0115. Tepckuu琼脂

Dissolve 0.83 g of sodium dibasic phosphate

(anhydrous) in 70ml distilled water

Dissolve 0.27 g of potassium monobasic

phosphate in 30 ml distilled water.

Make to 1.0 L with distilled water

pH    7.2

Add 8% sterile rabbit blood before use.

0116. Hottinger琼脂

Hottinger digest   1.0 L

NaCl   5.0 g

Na2HPO4   0.2 g

Agar    15.0 g

pH  7.0

[note] Hottinger digest:

Beef   500.0 g

Pig pancreas   100.0 g

Chloroform   20-25 ml

Distilled water  1.0 L

0117. Loeffler血清琼脂

Nutrient broth (containing 1% glucose) 100.0 ml

Sterile horse serum    300.0 ml

Sterilize intermittenttly in coagulator and make to slant

0118. Pope琼脂

Pope digest    1.0 L

Sodium acetate   5.0 g

Solution No.2  2.0 ml

Agar  15.0 g

pH  7.8-8.0

[note] Pope digest:

Beef   100.0 g

Pancreas   2.0 g

Acetic acid glacial   12.0 ml

Solution No.2:

Tobacco  1.15 g

1% copper sulfate   50.0 ml

1% MnCl2   15.0 ml

1% ZnSO4   40.0 ml

HCl   30.0 ml

Heptanedioic acid   0.075 g

Alanine  1.15 g

Distilled water   870.0 ml

0119. 马铃薯甘油琼脂

Potato glycerol infusion   250.0 ml

NaCl   8.5 g

Agar  15.0 g

Distilled water   750.0 ml

pH nature

0120. 硫乙醇酸钠培养基

Yeast extract   5.0 g

Peptone  15.0 g

L-cyctine     0.5 g

pH  7.0-7.2

0121. MTS琼脂

Meat trypsinized broth   500.0 ml

Meat infusion   500.0 ml

NaCl   5.0 g

Glucose   5.0 g

Na-thioglycerollate   0.5 g

Crumbled meat  10.0 g

Agar  15.0 g

pH  7.2-7.4

0122. GC琼脂

Hottinger digested (containing amino acid

150mg/100ml)   100.0 ml

Glucose  10.0 g

NaCl   5.0 g

L-cysteine   1.0 g

Agar  15.0 g

Distilled water   900.0 ml

pH  6.8-7.2

[note] Cool to 45, 5ml of defibrillated blood

were added.

0123. Sauton 培养基

Asparagine   4.0 g

Citric acid   2.0 g

K2HPO4   0.5 g

MgSO4·7H2O   0.5 g

Ferric ammonium citrate  0.05 g

Glycerol   60.0 ml

Distilled water   900.0 ml

0124. 蛋黄培养基

Yolk  60 ml

Physiological salt solution.  40 ml

Coagulated at 80 for 1 hour

0125. Lowensten-Jensen鸡蛋琼脂

Fresh eggs  15-16

Distilled water   375.0 ml

2% malachite green   10.0 ml

KH2PO4   1.5 g

MgSO4·7H2O   0.375 g


NaCl   2.5 g

Glucose   5.0 g

Na-thioglycerollate   0.5 g

Agar   8.0 g

Distilled water   1.0 L

asparagine  2.25 g

Glycerol  7.5 ml

Potato powder  18.8 g

0126. 胆汁马铃薯培养基

Fresh ox bile   95.0 ml

Glycerol  5.0 ml

Potato block (cylinder)

Preparation: Immerse the popato block in

bileglycerol solution at 75 for 30 min then

0132. Hottinger血琼脂

Hottinger digest (containing amino nitrogen

2mg/ml)   1.0 L

NaCl   5.0 g


put them into tubes.

Agar  15.0 g


0127. 类鼻疽菌培养基

Peptone  10.0 g

NaCl   5.0 g

Beef extract   3.0 g

Glycerol   40.0 ml

Agar  15.0 g

Distilled water   1.0 L

pH  7.0-7.2

0128. 半固体碎肉培养基

Meat trypsinized broth   500.0 ml

Beef infusion broth   500.0 ml

Glucose   5.0 g

Gelatin   4.0 g

Agar  15.0 g

pH   8.2

Crumbled beef (just the right amount in tube)

0129. 胱氨酸血琼脂

Beef infusion broth   1.0 L

Peptone  20.0 g

NaCl   5.0 g

Cysteine    1.0 g

Defibrillated rabbit blood   50.0 ml

Glucose  10.0 g

Agar  15.0 g

pH   7.4-7.6

0130. 3%NaCl脑心琼脂

Proteose  10.0 g

Yeast extract   5.0 g

NaCl  30.0 g

Na2HPO4   2.5 g

Brain infusion   400.0 ml

Ox infusion   600.0 ml

0131. 蔗糖培养基

Sucrose   100.0 g

Peptone   2.5 g

Na2HPO4   1.5 g

Agar  15.0 g

Distilled water   1.0 L

pH   7.0-7.2

pH   7.0

Cool to 45,10 ml of 10% blood were added.

[note] (1) Hottinger digest: see medium 116.

(2) 10% blood (hemolysis): 10ml of

defibrillated blood add to 90ml of distilled

water.

0133. 猪胃胨半流体培养基

Acid digest swine stomach broth  500.0 ml

Ox heart infusion   500.0 ml

NaCl   2.5 g

Glucose  10.0 g

Argine   2.5 g

Agar    4.0 g

pH   7.6-7.8

Cool to 45-56, then add horse serum 2 ml,

25% fresh yeast infusion 1ml and ammonium

citrate 0.25 mg/ml and penicillin 200 ug/ml

0134. 麸皮培养基2

Wheat bran  70.0 g

Agar  15.0 g

Distilled water   1.0 L

pH nature

0135. 麸皮培养基3

Wheat bran  30.0 g

Agar  15.0 g

Distilled water   1.0 L

pH nature

0136. 麸皮培养基4

Wheat bran  50.0 g

(NH4)2HPO4  0.05 g

Agar    15.0 g

Distilled water   1.0 L

pH nature

0137. 豌豆培养基

Glucose   10.0 g

(NH4)2SO4  10.0 g

NaCl   5.0 g

Peptone   5.0 g

Pea infusion   1.0 L

Agar   15.0 g

pH   7.0-7.2

0138. 拉氏培养基

Glycerol  15.0 g

Corn steep liquor   2.5 g

Peptone   6.0 g

Glucose   10.0 g

Peptone   3.0 g

NaCl   2.5 g

CaCO3   2.0 g

Agar  15.0 g

Distilled water   1.0 L


NaCl   5.0 g

KCl   0.5 g

KH2PO4   0.3 g

MgSO4·7H2O  0.05 g

CuSO4·5H2O  0.02 g

FeSO4·7H2O    0.015 g

MnSO4·7H2O  0.02 g

Agar  15.0 g

Distilled water   1.0 L

pH   7.0

0139. 莫氏培养基

Lactose  15.0 g

Corn steep liquor   2.5 g

Peptone   5.0 g

MgSO4·7H2O  0.05 g

KH2PO4  0.05 g

NaCl   4.0 g

CuSO4·5H2O   0.004 g

Agar  15.0 g

Distilled water   1.0 L

0140. 蚕蛹粉培养基

Silkworm chrysalis powder   3.0 g

Glucose  10.0 g

Soybean cake meal  10.0 g

NaCl   2.5 g

CaCO3   2.0 g

Agar  15.0 g

Distilled water   1.0 L

0143. 麸皮培养基5

Wheat bran  36.0 g

(NH4)2SO4   3.0 g

K2HPO4   0.2 g

MgSO4·7H2O    0.1 g

Agar  15.0 g

Distilled water   1.0 L

pH   7.0

0145. 可可培养基

Brain/hearts infusion (Difco)   1.0 L

Nicotinamide adenine dinucleotide  2.0 mg

Hemin   0.01 mg

Ager    15.0 g

0146. PYE培养基

植物蛋白胨   5.0 g

酵母提取物   2.5 g

K2HPO4   3.7 g

KH2PO4   1.3 g

MgSO4·7H2O   0.5 g

NaCl   1.0 g

琼脂  15.0 g

蒸馏水   1.0 L

pH  7.2-7.5

0147. TY培养基

酪蛋白   5.0 g

酵母提取物   3.0 g

CaCl2·6H2O   1.3 g

琼脂  15.0 g


pH    7.2-7.4

0141. BP琼脂

Beef extract  10.0 g

Peptone  10.0 g

NaCl   5.0 g

Agar  15.0 g

Distilled water   1.0 L

pH  7.0-7.2

0142. GPS琼脂

蒸馏水   1.0 L

pH  7.0

0148. 海水培养基

(1) The artificial sea water (ASW)

NaCl   0.4M

MgSO4·7H2O   0.1M

KCl  0.02M

CaCl2·2H2O  0.02M

(2) The basal medium (BM)


Soybean cake powder  10.0 g

tris (hydroxy methyl) aminomethane (tris)-

hydrochloride (pH7.5)  50mM

NH4Cl   190 mM

K2HPO4·3H2O   0.33mM

FeSO4·7H2O   0.1mM

Half strength ASW

(3) Basal medium agar (BMA)

Casein (or peptone)  10.0 g

Agar  15.0 g

Distilled water   1.0 L

0153. MPY培养基

Maltose  30.0 g

Corn meal  10.0 g


Was prepared by separately sterilizing and

then mixing equal volumes of double strength

Yeast extract   3.0 g

Peptone  10.0 g


BM add 15 g agar per liter.

Agar  15.0 g


(4) Yeast extract broth (YEB)

Was made by supplementing BM with 5 g of

Difco yeast extract per liter

(5) Yeast extract agar (YEA)

To be prepared by solidifying YEB with 15 g

of Difco Agar per liter

0149. 燕麦琼脂-2

燕麦粉  30.0 g

Distilled water     1.0 L

pH   6.8

0154. PPDA培养基

Peptone  10.0 g

Potato   200.0 g

Glucose  20.0 g

Agar  15.0 g

Distilled water    1.0 L


琼脂  15.0 g

蒸馏水   1.0 L

0150. MMN培养基

Malt extract   3.0 g

Glucose  10.0 g

Peptone  15.0 g

CaCl2  0.05 g

MgSO4·7H2O  0.15 g

NaCl   0.025 g

FeCl3 (1%)  1.2 ml

KH2PO4   0.5 g

(NH4)2HPO4  0.25 g

Viatmin B1   1.0 mg

Agar  15.0 g

Distilled water   1.0 L

pH    5.5-5.7

0151 PDMA培养基

Potato infusion (20%)   500.0 ml

Wort (2Be)   500.0 ml

Glucose  20.0 g

Vitamin B1    0.05 g

Agar  15.0 g

pH   6.5

0155. BPG琼脂

Beef extract   3.0 g

Peptone   8.0 g

Sodium citrate   5.0 g

Agar  15.0 g

Distilled water   1.0 L

pH   7.2

0156. PG琼脂

Peptone  15.0 g

Glucose  15.0 g

Agar  15.0 g

Distilled water   1.0 L

0158. YEM琼脂

Manitol  10.0 g

Yeast extract   0.8 g

K2HPO4    0.5 g

NaCl   0.2 g

MgSO4·7H2O   0.2 g

CaCO3   5.0 g

0.5% NaMoO4  4.0 ml

0.5% H3BO3  4.0 ml


pH   5.5-6

0152. MGYC培养基

L-Methionine   0.2 g

Glucose  10.0 g

Yeast extract   5.0 g

Agar   15.0 g

Distilled water   1.0 L

0160. 大豆芽汁培养基

Bean sprouts broth   1.0 L

Sucrose  50.0 g

Agar  15.0 g

pH nature

Peptone  10.0 g

MgSO4·7H2O    0.15 g


[note]: Soybean 100g, add water 1.0 L, boiling

for 30 min., filtrate.

KH2PO4   2.0 g

Agar  15.0 g


0161. 醋酸杆菌培养基

3 Beaume wort   1.0 L

Yeast extract   5.0 g

Glucose  20.0 g

MgSO4·7H2O   2.0 g

K2HPO4   3.0 g

Agar  15.0 g

pH nature

[note]: After sterilization, add 95% ethanol to

make the conc. of the ethanol in broth into 2-3%.

0162. 丙酮丁醇梭菌培养基

(1) Mixing corn meal and water into paste, putting the paste into boiled water, then boiling and stiring for 1 hr., making it in5% concentration, distributing into tubes, autoclave at 1.5kg/cm square for 90 min. for pastification

(2) Inoculation with bacteria spore in sand when cold

(3) After inoculation, putting the tubes in boiling water bath for 1-2 min, for heat treatment

(4) Cooling the tubes in top water immediately

(5) Putting the tubes in vacuum desicator and pumping it to vacuum

(6) Incubation at 37C for 3 days, when the surface of the broth floated with soild material and the cloud-like suspended in

the lower part of the tube, the cultures are growth well

0163. 纤维发酵细菌培养基

5 Beaume wort   1.0 L

Peptone   0.5 g

(NH4)2HPO4   0.1 g

CaCO3 (sterilized)     0.6 g

Agar  15.0 g

pH   7.0

0164.

Glucose  10.0 g

Distilled water    1.0 L

pH   5.6

0165. 丙酸细菌培养基

Glucose  20.0 g

Na2HPO4   1.0 g

Lactose  20.0 g

MgSO4·7H2O   0.4 g

Yeast extract  10.0 g

Glycerol    1.0 g

Agar  15.0 g

Distilled water   1.0 L

pH   6.8-7.0

0166. 橄榄色链霉菌培养基

Wheat bran  50.0 g

Agar    15.0 g

Distilled water   1.0 L

pH   7.0

[note]: Taking 50 g wheat bran in 1.0 L water,

immersing it in water bath at 55-60 for 4-5

hours, then boiling for 30 min., making the

volume into 1.0 L with water.

0167. 明串珠菌培养基

Sucrose    125.0 g

Peptone   4.0 g

K2HPO4    0.4 g

NaHCO3    1.5 g

Manganese chloride   30.0 ppm

Agar  15.0 g

Water   1.0 L

pH   7.0

0168.

Urea   3.0 g

Corn steep liquor   1.0 g

KH2PO4   2.0 g

MgSO4·7H2O   1.0 g

MnSO4·4H2O  0.02 g

FeSO4·7H2O   0.2 g

Distilled water    1.0 L

pH nature

[note]: After sterilization, add the sterile

paraffin oil.

0169. 酒明串珠菌培养基

Glucose  10.0 g

Peptone    10.0 g

Yeast extract   5.0 g

Tomato juice   250.0 ml

MgSO4·7H2O   0.2 g

MnSO4·4H2O  0.05 g

Distilled water   750.0 ml

pH   4.8

[note]: Each tube distributed the broth 10ml, then sterilized. Before inoculation, 0.5 ml of 1% cysteine phosphate added to each tube.

0170. 纸浆发酵细菌培养基

Peptone   5.0 g

Yeast extract   3.0 g

Glucose   5.0 g

K2HPO4   1.0 g

NaCl   5.0 g

Agar  15.0 g

Distilled water   1.0 L

pH   8-9

0171. 牛肝浸液培养基

Diced beef liver   500.0 g

Peptone  10.0 g

(NH4)2SO4   0.5 g

[note]: After sterilization add ethanol (the total

conc. in 2-2.5%).

0173.

NaNO3   2.0 g

MgSO4·7H2O   0.5 g

Yeast extract   2.0 g

Agar  15.0 g

Tap water   1.0 L

pH   7.0

0174. 嗜热脂肪芽孢杆菌培养基1

Yeast extract  10.0 g

Tryptose  10.0 g

K2HPO4  10.0 g

MnSO4  0.03 g

Agar   15.0 g

Distilled water   1.0 L

pH   7.0

0175. 嗜热脂肪芽孢杆菌培养基2

Beef extract  10.0 g

Peptone  10.0 g

Glucose  10.0 g

NaCl   5.0 g

K2HPO4   2.5 g

Agar  15.0 g


K2HPO4   1.0 g

Water   1.0 L

[note]: (1) Putting 500g of the diced and de-fat

beef liver in 1.0 L water, immersed at

refrigerator over night, autoclave for 10 min.,

filtration by suction.

(2) Adding 10 g of peptone and 1g of

K2HPO4, adjusting pH to 8.0, filtrating with

paper, making the volume into 1.0 L.

(3) The beef liver residue retained.

(4) Distributing the broth into tube up to 1 in. then adding the beef liver residue in tube up to 2  in.(total), and CaCO3  in trace.

0172. 己酸细菌培养基

Yeast extract   1.0 g

Sodium acetate   0.5 g

MgSO4·7H2O  0.02 g

K2HPO4   0.4 g

CaCO3   0.5 g

Distilled water   1.0 L

1.6% Bromocresol purple  1.0 ml

0176. 凝结芽孢杆菌培养基

Yeast extract   5.0 g

MnSO4   0.03 g

Tryptose   5.0 g

K2HPO4   5.0 g

Agar  15.0 g

Distilled water   1.0 L

pH   6.2

0177. 节杆菌培养基

Glucose  20.0 g

K2HPO4   2.0 g

Yeast extract   1.5 g

(NH4)2HPO4   6.0 g

MgSO4·7H2O   0.1 g

Agar  15.0 g

Distilled water   1.0 L

pH   7.0-7.2

0178.

Glucose  20.0 g

Urea   2.0 g

Yeast extract   4.0 g

Agar  15.0 g

Distilled water   1.0 L

pH   4.5

0179. 肌苷细菌培养基

Glucose  30.0 g

NaCl   3.0 g

Peptone   1.0 g

Agar   2.0 g

Yeast extract   1.0 g

Agar  15.0 g

Distilled water   1.0 L

0180.

Glucose  30.0 g

pH   7.2

Add 10 ml of chicken serum and 10ml of 25%

yeastextract before use

0184. PPLO琼脂

PPLO Agar (Difco)   1.0 L

pH   7.2

Add 1% NADH solution 10ml, yeast extract

2.5 g, horse serum 5 ml and glucose 0.1 g

when the medium cold to 56.

0185. 改良Minca培养基

KH2PO4  1.36 g

Na2HPO4   8.0 g

Yeast extract   1.0 g

Casein peptone   5.0 g

Glycerol  5.0 ml

trace salt solution  1.0 ml

Agar  15.0 g


Casein hydrolyzate  30.0 g

Agar  15.0 g

Distilled water   1.0 L

0181. 酵母菌培养基

Yeast extract   3.0 g

Malt extract   3.0 g

Peptone   5.0 g

Glucose  10.0 g

Agar  15.0 g

Distilled water   1.0 L

0182. PY培养基

Potato infusion   1.0 L

L-asparagine  10.0 g

KH2PO4  10.0 g

MgSO4·7H2O   0.5 g

Ferrum citrate   1.2 g

Mycobacillin   1.0 g

Glycerol   60.0 ml

Tween 80   15.0 ml

Agar  15.0 g

0183. 鸡血清鸡肉汁培养基

Chicken broth   1.0 L

Polypeptone   5.0 g

Tryptose   5.0 g

Glutamate   5.0 g

NaCl   5.0 g

Agar  15.0 g

Distilled water    1.0 L

Trace salt solution:

MgSO4·7H2O  10.0 g

MnCl2·4H2O   1.0 g

FeCl2·6H2O   0.135 g

CaCl2·2H2O   0.4 g

Distilled water   1.0 L

0186. FREY培养基

Basic solution:

NaCl   2.5 g

KCl   0.2 g

Na2HPO4   0.8 g

KH2PO4  0.05 g

MgSO4·7H2O   0.1 g

Casein hydrolysed   2.5 g

Glucose   5.0 g

De-ionic water   500.0 ml

After sterilized, add 60ml of sterile pig serum, 0.0625 g of thallium acetate, 1g of argininate, 50 ml of 25% yeast extract, 0.05g of phenol red and 50 units of penicillin. Adjust ph to 7.6-7.7 by 1N NaOH.

0187. 改良FREY培养基

In the formula of Freys medium (0186), remove the argininate but add 13.0ml of pig serum.

0188. A62培养基

Hanks casein solution   500.0 ml

Beef heart infusion   300.0 ml

Sterile pig serum   200.0 ml

25% yeast extract  5.0 ml

Penicillin   25 units 25.0 Unit

Thallium acetate   0.00125 g

pH  7.5-7.8 by 1N NaOH

[note]: Prepare the medium aseptically

0189. 蚕豆芽培养基

Bean sprouts infusion   1.0 L

Glucose  50.0 g

Agar    15.0 g

pH nature

0190. 糖浆培养基

5 Be Molasses solution   1.0 L

Urea   1.0 g

Agar  15.0 g

pH   5-5.6

0191.

Smilax glabra root infusion   300.0 ml

5 Be wort   700.0 ml

Agar  15.0 g

Distilled water   1.0 L

pH nature

0195. 番茄汁培养基

Yeast extract   7.5 g

Peptone   7.5 g

Glucose  10.0 g

K2HPO4   2.0 g

Tomato juice   100.0 ml

Tween 80  0.5 ml

CaCO3  10.0 g

Agar  15.0 g

Distilled water   900.0 ml

pH   7.0

0196. 葡萄汁培养基

Grape juice   500.0 ml

Yeast extract   0.5 g

Tween 80  0.5 ml

Distilled water   500.0 ml

pH nature

0197.


Agar  15.0 g

pH nature

[note] Add 1.0 L of water into 250 g of Smilax glabra root in the beaker, boil for 2-3 hours, filtrate and make into 1.0 L.

0192. 曲霉产孢培养基

3 Be Wort   1.0 L

NaCl  30.0 g

Agar  15.0 g

pH   4-6

0193. 麸皮培养基6

5% wheat bran infusion   1.0 L

FeSO4 ·7H2O  200.0 ppm

Agar  15.0 g

pH   7.0

0194. 龟裂链霉菌培养基

Soluble starch  60.0 g

NaCl   5.0 g

Peanut cake meal  20.0 g

KH2PO4   0.1 g

Corn steep liquor   5.0 g

CaCO3   8.0 g

(NH4)2SO4   8.0 g

Glucose   1.0 g

Yeast extract   3.0 g

Agar  15.0 g

Distilled water   1.0 L

pH    7.0

0198.

Bacto-peptone  10.0 g

Bacto-yeast extract   5.0 g

NaCl  10.0 g

Agar  15.0 g

Distilled water   1.0 L

pH   7.5

0199.

Glucose   100.0 g

Yeast extract   5.0 g

(NH4)2SO4   1.0 g

KH2PO4    1.0 g

MgSO4·7H2O   0.5 g

Agar  15.0 g

Distilled water   1.0 L

0200. 综合麦芽汁培养基

Potato infusion (20%)   900.0 ml

MgSO4·7H2O   1.5 g

KH2PO4   3.0 g

Wort   100.0 ml

Thamine trace trace

Glucose  20.0 g

Agar   15.0 g

pH   6.0

0201. 综合麦芽糖培养基

20% Potato broth   1.0 L

Maltose  50.0 g

KH2PO4   3.0 g

MgSO4·7H2O   1.5 g

Agar  15.0 g

Adjust pH to 5.5 with citric acid.

0202.

Glucose  20.0 g

MgSO4·7H2O   0.1 g

Sodium cholate   3.0 g

K2HPO4   2.5 g

Glycerol   30.0 ml

NaCl  15.0 g

Agar  15.0 g

Distilled water   1.0 L

0206. YPG培养基

酵母提取物   5.0 g

蛋白胨   5.0 g

葡萄糖   5.0 g

琼脂  15.0 g

蒸馏水   1.0 L

pH   7.2

0207. NBY培养基


Beef extract  10.0 g

Birch sawdust infusion   1.0 L

Agar  15.0 g

pH natural

[note] Take 300 g of birch sawdust into 1.0 L of water, immerse it for 16 hours, then boil for 20 min., filtrate and make the filtrate into 1.0  L.

0203.

20% Potato broth   1.0 L

Sucrose  10.0 g

Glucose  10.0 g

KH2PO4   3.0 g

MgSO4·7H2O   1.5 g

Yeast extract   2.0 g

Agar  15.0 g

pH nature

0204. 黑色素培养基

Yeast extract   1.0 g

NaCl   15.0 g

Glycerol   30.0 ml

1.0% melanin pigment solution   20.0 ml

Agar  15.0 g

Fungicidin   0.2 g

Distilled water   950.0 ml

0205. IVANOV培养基

Ammonium iron citrate  10.0 g

CaCl2  0.01 g

Na2SO4   2.5 g

Beef extract   8.0 g

Yeast extract   2.0 g

K2HPO4   2.0 g

KH2PO4   0.5 g

Agar  15.0 g

Distilled water   1.0 L

[note] Add 50 ml of 10% glucose solution and 1 ml of 1M MgSO4.7H2O solution aseptically after sterilization.

0208. TZC培养基

Beef extract   5.0 g

Glucose  10.0 g

Yeast extract   0.5 g

Peptone  10.0 g

Agar  15.0 g

Distilled water   1.0 L

After sterilization, add 0.5 ml of the 1% TZC (2,3,5-nitrogen chloride-phenyl-tetrazole) solution to 100 ml of the medium.

0209. 可可培养基

脑心琼脂   1.0 L

辅酶Ⅱ   2.0 mg

高铁血红素   10.0 mg

0210. R琼脂

蛋白胨  10.0 g

酵母提取物   5.0 g

麦芽提取物   5.0 g

酪蛋白氨基酸   5.0 g

牛肉提取物   2.0 g

甘油   2.0 g

Tween 80   50.0 mg

MgSO4·7H2O   1.0 g

琼脂   18.0 g

蒸馏水  1.0 L

pH  7.2

0211.

酪蛋白氨基酸   7.5 g

酵母提取物  10.0 g

柠檬酸钠   3.0 g

MgSO4·7H2O   0.1 g

KCl   2.0 g

Fe++   微量

Mn++   微量

NaCl   200.0 g

Na2CO3 (单独灭菌)   8.0 g

琼脂  15.0 g

蒸馏水   1.0 L

pH  9.5

0212. CM琼脂

酪蛋白氨基酸   7.5 g

酵母提取物  10.0 g

琼脂  15.0 g

蒸馏水   1.0 L

每个斜面加液体石蜡一滴

pH  7.0

0217. 脑心琼脂

心提取物   5.0 g

脑提取物  12.5 g

示胨  10.0 g

葡萄糖   2.0 g

Na2HPO4   2.5 g

琼脂  15.0 g

蒸馏水   1.0 L

pH   7.4

0218. PS琼脂

蛋白胨  10.0 g

蔗糖  20.0 g

琼脂  15.0 g

蒸馏水   1.0 L

pH   7.0

0219.

LB琼脂   1.0 L

氨苄青霉素   50.0 mg


柠檬酸钠    3.0 g

MgSO4·7H2O  20.0 g

KCl   2.0 g

Fe++   10.0 ppm

NaCl   200.0 g

琼脂  15.0 g

蒸馏水   1.0 L

pH  7.2

0213. 血琼脂-2

血琼脂基础培养基   1.0 L

脱纤维兔血   5%

0214. 血琼脂-3

Trypticase Soy 琼脂   1.0 L

脱纤维兔血   5%

0215. GAM琼脂

GAM琼脂  74.0 g

蒸馏水   1.0 L

0216.

蛋白胨  10.0 g

酵母提取物  10.0 g

牛肉提取物   3.0 g

NaCl   5.0 g

0220. M9培养基

Na2HPO4   6.0 g

KH2PO4   3.0 g

NaCl   0.5 g

NH4Cl   1.0 g

0.01M CaCl   10.0 ml

1M MgSO4  1.0 ml

20% Glucose   10.0 ml

硫胺素    1.0 mg

氨基酸蛋白水解液   80.0 mg

Distilled water  1.0 L

pH   7.0

0221. 胰胨、大豆胨琼脂

Trypticase Soy 琼脂  40.0 g

蒸馏水   1.0 L

0222.

蔗糖  15.0 g

K2HPO4   0.8 g

KH2PO4   0.2 g

MgSO4·7H2O   0.2 g

NaCl   0.2 g

CaCl2   0.05 g

MnSO4   0.0005 g

FeSO4   0.025 g

钨酸钠   0.0005 g

钼酸钠   0.0005 g

琼脂  15.0 g

蒸馏水   1.0 L

自然pH

0223. 海水2216琼脂

海水2216琼脂    55.1 g

蒸馏水   1.0 L

pH   7.4

0224.

NaCl   30.0 g

K2HPO4     3.9 g

KH2PO4   2.1 g

NH4Cl   5.0 g

酵母提取物   5.0 g

Tryptone   5.0 g

1 M Tris Buffer (pH7.5)   50.0 ml

甘油  3.0 ml

MgSO4·7H2O   1.0 g

KCl  0.75 g

CaCO3   1.0 g

琼脂  15.0 g

蒸馏水   1.0 L

pH    7.2

0225.

牛肉提取物   5.0 g

酵母提取物   1.0 g

蛋白胨   5.0 g

蔗糖   5.0 g

1M MgSO4·7H2O  2.0 ml

琼脂  15.0 g

蒸馏水   1.0 L

pH   7.0

0226.

蔗糖   5.0 g

蛋白胨   5.0 g

酵母提取物   1.0 g

牛肉提取物   3.0 g

琼脂  15.0 g

蒸馏水   1.0 L

pH   7.0

LB琼脂   1.0 L

四环素   50.0 mg

0228.

Trytone  10.0 g

酵母提取物   5.0 g

葡萄糖   1.0 g

维生素B1  20 mg

NaCl   5.0 g

琼脂  15.0 g

蒸馏水   1.0 L

0229.

牛肉提取物   5.0 g

酵母提取物   2.0 g

蛋白胨  10.0 g

NaCl   2.0 g

Neomycin   5.0 mg

琼脂  15.0 g

蒸馏水   1.0 L

pH   6.8

0230.

酵母提取物   5.0 g

蛋白胨   3.0 g

甘露醇  25.0 g

琼脂  15.0 g

蒸馏水   1.0 L

0231.

蛋白胨  10.0 g

牛肉提取物  10.0 g

酵母提取物   5.0 g

葡萄糖   5.0 g

K2HPO4  0.45 g

KH2PO4  0.33 g

NH4Cl   1.0 g

MgSO4·7H2O   0.1 g

5% L-Cysteine.HCl H2O solution  10.0 ml

0.1% Resazurin solution  1.0 ml

5% Na2S·9H2O solution   10.0 ml

蒸馏水   1.0 L

pH   7.5

0232. 3%NaCl营养琼脂

营养琼脂   1.0 L

NaCl   3%

0233. Bifidobacterium medi


0227. 四环素LB琼脂

大豆蛋白胨   5.0 g

胰胨   5.0 g

酵母粉  10.0 g

Glucose    10.0 g

Trace salt solution   40.0 ml

L-Cysteine-HCl·H2O   0.5 g

0.1%刃天青  1.0 ml

Agar  15.0 g

Dist.water   1.0 L

pH   7.0

Trace salts solution:

CaCl2   0.2 g

MgSO4·7H2O  0.48 g

K2HPO4   1.0 g

KH2PO4   1.0 g

NaHCO3  10.0 g

NaCl   2.0 g

蒸馏水   1.0 L

pH   6.5

0234.

蛋白胨  10.0 g

酵母提取物   2.0 g

水解酪素   2.0 g

NaCl   6.0 g

葡萄糖  10.0 g

琼脂  18.0 g

蒸馏水   1.0 L

pH   7.2

0235.

蛋白胨  10.0 g

酵母提取物   2.0 g

水解酪素   2.0 g

NaCl   6.0 g

琼脂  15.0 g

蒸馏水   1.0 L

pH   7.2

0236. CYC琼脂

Czapek-Dox powder  33.4 g

酵母提取物   2.0 g

酪蛋白氨基酸   6.0 g

琼脂  15.0 g

蒸馏水   1.0 L

pH   7.2

0237. 碱性ISP-2培养基

Medium No.0038, after autoclaving,

adjust pH to 10.0 with sterilized 10% Na2CO3 solution.

0238.

Polypeptone   5.0 g

Yeast extract   5.0 g

Glucose   5.0 g

Lactose   2.0 g

Tween 80   0.5 g

MgSO4·7H2O   1.0 g

Agar  15.0 g

Distilled water   1.0 L

pH   6.5-6.8

0239.

Yeast extract   1.0 g

Beef extract   1.0 g

N-Z amine (type A)   2.0 g

Maltose  10.0 g

Agar  15.0 g

Distilled water   1.0 L

pH   7.3

0240.

Glucose  10.0 g

L-Asparagine   1.0 g

K2HPO4   0.5 g

Yeast extract   2.0 g

Agar  15.0 g

Distilled water   1.0 L

pH   7.3

0241. 3%NaClR琼脂

Medium No. 0210   1.0 L

NaCl  30.0 g

0242.

Potato   200.0 g

Yeast extract   2.0 g

Glucose  20.0 g

Distilled water   1.0 L

pH    5.6

0243. 改良Horikoshii2

多胨   5.0 g

酵母提取物   5.0 g

淀粉  10.0 g

KH2PO4   1.0 g

MgSO4·7H2O   0.2 g

NaCl  10.0 g

琼脂  15.0 g

蒸馏水   1.0 L

pH   8.0

0244. PYG培养基

多胨   5.0 g

胰胨   5.0 g

酵母提取物  10.0 g

葡萄糖  10.0 g

盐溶液   40.0 ml

蒸馏水   960 ml

琼脂  10.0 g

pH   7.2

盐溶液:

CaCl   0.2 g

MgSO4·7H2O   0.4 g

K2HPO4   1.0 g

KH2PO4   1.0 g

NaHCO3  10.0 g

NaCl  12.0 g

蒸馏水   1.0 L

0245.

酪蛋白氨基酸   7.5 g

酵母提取物  10.0 g

柠檬酸钠   3.0 g

MgSO4·7H2O  20.0 g

KCl   2.0 g

NaCl   200.0 g

Fe++  0.05 g

琼脂  15.0 g

蒸馏水   1.0 L

pH   7.4

0246.

酪蛋白氨基酸   7.5 g

酵母提取物  10.0 g

柠檬酸钠   3.0 g

MgSO4·7H2O  20.0 g

KCl   2.0 g

NaCl   150.0 g

微量盐溶液  1.0 ml

琼脂  15.0 g

蒸馏水   1.0 L

pH  7.5

微量盐溶液:

FeSO4·7H2O   0.1 g

MnCl2·4H2O   0.1 g

ZnSO4·7H2O   0.1 g

蒸馏水   100 ml

0247.

酪蛋白氨基酸    7.5 g

酵母提取物  10.0 g

柠檬酸钠   3.0 g

MgSO4·7H2O  20.0 g

KCl   2.0 g

NaCl   200.0 g

微量盐溶液  1.0 ml

琼脂  15.0 g

蒸馏水    1.0 L

pH  7.5

微量盐溶液:

FeSO4·7H2O   0.1 g

MnCl2·4H2O   0.1 g

ZnSO4·7H2O   0.1 g

蒸馏水   100 ml

0248. 酵母粉、淀粉琼脂-2

可溶性淀粉  15.0 g

酵母提取物   4.0 g

K2HPO4   0.5 g

MgSO4·7H2O   0.5 g

琼脂  15.0 g

蒸馏水   1.0 L

pH   7.4

0249. 酵母粉燕麦琼脂

燕麦琼脂(0039号培养基)    1.0 L

酵母提取物   1.0 g

0250. 6B培养基

酵母提取物    5.0 g

甘油  50.0 g

N-Z amine (Type A)   5.0 g

葡萄糖  10.0 g

可溶性淀粉  20.0 g

CaCO3   1.0 g

琼脂  15.0 g

蒸馏水   1.0 L

自然pH

0251. N-Z Amine A培养基

N-Z amine(Type A)   5.0 g

牛肉提取物   1.0 g

甘油   80.0 ml

琼脂   15.0 g

蒸馏水   1.0 L

pH   7.0

0252. 硝酸盐燕麦琼脂

燕麦粉   3.0 g

KNO3   0.2 g

K2HPO4   0.5 g

MgSO4·7H2O   0.2 g

琼脂  15.0 g

蒸馏水   1.0 L

pH   7.0

0253. MGA琼脂

葡萄糖   2.0 g

L天门冬素   1.0 g

K2HPO4    0.5 g

MgSO4·7H2O   0.5 g

微量盐溶液  1.0 ml

琼脂  15.0 g

蒸馏水   1.0 L

pH   7.4

微量盐溶液:

FeSO4·7H2O    1.0 g

CuSO4·5H2O   0.1 g

ZnSO4·7H2O   0.1 g

蒸馏水   100.0 ml

0254.

KH2PO4   1.0 g

KCl   1.0 g

NH4Cl   1.0 g

MgSO4·7H2O  0.24 g

CaSO4·2H2O  0.17 g

NaCl   200.0 g

Trace salt solution  1.0 ml

谷氨酸钠   1.0 g

酵母提取物   5.0 g

酪蛋白氨基酸   5.0 g

Na2CO3 (单独灭菌)   5.0 g

琼脂  20.0 g

蒸馏水   1.0 L

CoCl2·6H2O   0.2 g

CuCl2·2H2O  0.01 g

NiCl2·6H2O  0.02 g

Na2MoO4·2H2O  0.03 g

蒸馏水   1.0 L

0255. 西红柿培养基

西红柿酱  20.0 g

葡萄糖  20.0 g

酵母提取物  10.0 g

CoCl2·6H2O   5.0 mg

琼脂  15.0 g

蒸馏水   1.0 L

pH   7.2-7.4

0256.

麦芽提取物    1.0 g

酵母提取物   1.0 g

肝提取物   1.0 g

葡萄糖   5.0 g

蔗糖   2.0 g

琼脂  15.0 g

蒸馏水   1.0 L

pH   7.0

0257.

山梨糖  20.0 g

Yeast extract   3.0 g

Peptone  10.0 g

MgSO4·7H2O   0.2 g

KH2PO4    1.0 g

CaCO3   1.0 g

尿素   1.0 g

玉米浆   3.0 g

Agar  15.0 g

Distilled water   1.0 L

pH   7.0-7.2

0258.

Yeast extract   3.0 g

peptone   5.0 g

glucose   5.0 g

Soluble starch   1.5 g


pH   7.4

Tracesalt solution:

ZnSO4·7H2O   0.1 g

MnCl2·4H2O  0.03 g

H3BO3   0.3 g

Sucrose   5.0 g

Beef extract   2.0 g

K2HPO4   2.0 g

NaCl   3.0 g

Agar  15.0 g

Distilled water   1.0 L

pH   6.8

0259. ATYP琼脂

KH2PO4   1.0 g

CaCl2   0.1 g

NaHCO3   3.0 g

Na-acetate   1.0 g

MgCl2   0.5 g

NH4Cl   1.0 g

NaCl   1.0 g

Trace element solution  1.0 ml

Vitamine solution  1.0 ml

琥珀酸钠   1.0 g

Yeast extract   0.5 g

peptone   0.5 g

Agar  15.0 g

Distilled water   1.0 L

pH   6.8

Trace element solution

FeCl2·4H2O   1.8 g

CoCl2·6H2O  0.25 g

NiCl2·6H2O  0.01 g

CuCl2·2H2O  0.01 g

MnCl2·4H2O   0.7 g

ZnCl2   0.1 g

H3BO3   0.5 g

Na2MoO4·2H2O  0.03 g

Na2SeO3·5H2O  0.01 g

蒸馏水   1.0 L

维生素溶液

生物素   0.1 g

烟酸  0.35 g

盐酸硫胺素   0.3 g

对氨基苯甲酸   0.2 g

盐酸吡哆胺   0.1 g

泛酸钙   0.1 g

维生素B12  0.05 g

蒸馏水   1.0 L

0260. 烷烃培养基

NH4NO3   5.0 g

K2HPO4   2.5 g

MgSO4·7H2O   1.0 g

Corn steep liquor   0.1 g

Agar  15.0 g

Tap water   1.0 L

pH   7.0

0261. 双歧杆菌培养基-2

胰酶水解酪蛋白  10.0 g

Meat extract   5.0 g

Yeast extract   5.0 g

Glucose   10.0 g

K2HPO4   3.0 g

Tween 80  1.0 ml

Vitamine C  10.0 g

L-cysteime·HCl   0.5 g

Agar  15.0 g

Distilled water   1.0 L

pH   6.8

0262. M9无机盐培养基

10×M9 salts   100.0 ml

1M MgSO4  1.0 ml

0.1M CaCl2  1.0 ml

1M Thiamine.HCl.2H2O  1.0 ml

Glucose(20%)   10.0 ml

Proline   20.0 mg

Distilled water   900.0 ml

pH   7.4

10×M9 salts:

Na2HPO4  60.0 g

KH2PO4  30.0 g

NH4Cl  10.0 g

NaCl   5.0 g

Distilled water   1.0 L

0263. 1%NaCl营养琼脂

Medium No. 0002 with 1% NaCl

0264. 改良PYG培养基


Trypticase peptone   5.0 g

Peptone   5.0 g

Yeast extract  10.0 g

Beef extract   5.0 g

Glucose   5.0 g

K2HPO4   2.0 g

Tween80  1.0 ml

Resazurin    1.0 mg

Salt solution   40.0 ml

Haemin solution   10.0 ml

Vitamine K1 solution  0.2 ml

Cys.HCl.H2O   0.5 g

Distilled water   950.0 ml

pH   7.2

Salt solution:

CaCl2·2H2O  0.25 g

MgSO4·7H2O   0.5 g

K2HPO4    1.0 g

KH2PO4   1.0 g

NaHCO3  10.0 g

NaCl   2.0 g

Distilled water   1.0 L

Haemin solution

氯化血红素   50.0 mg

1 N NaOH  1.0 ml

蒸馏水   99.0 ml

Vitamine K1 solution

维生素K1  0.1 ml

95%乙醇   20.0 ml

0265. PY滤纸培养基

蛋白胨   0.5 g

胰酶水解酪素   0.5 g

酵母提取物   1.0 g

盐溶液A    10.0 ml

盐溶液B   1.0 ml

维生素溶液    10.0 ml

L-半胱氨酸   0.5 g

刃天青   1.0 mg

蒸馏水   1.0 L

蒸馏水   1.0 L

维生素溶液:

生物素   0.02 mg

叶酸   2.0 mg

维生素B6   0.1 mg

维生素B2  0.05 mg

维生素B1   0.05 mg

烟酸   0.05 mg

对氨基苯甲酸   0.05 mg

维生素B12   0.1 mg

D-泛酸钙   0.05 mg

蒸馏水   100.0 ml

0266. 营养琼脂-1

Peptone   5.0 g

Meat extract   3.0 g

Agar  15.0 g

Distilled water   1.0 L

pH   7.0

0267.

NaCl  80.0 g

MgSO4·7H2O   9.5 g

KCl   0.5 g

CaCl2·2H2   0.2 g

(NH4)2SO4   0.1 g

KNO3   0.1 g

Yeast extract   1.0 g

Hutner s salts   20.0 ml


pH   7.0

滤纸     1/试管

盐溶液A

KH2PO4  20.0 g

Na2HPO4  30.0 g

NH4Cl  50.0 g

MgSO4·7H2O   5.0 g

CaCl2·2H2O  10.0 g

FeSO4·7H2O   2.0 g

蒸馏水   1.0 L

盐溶液B

MnSO4·H2O   5.0 g

ZnSO4·7H2O   1.0 g

CoCl2·6H2O   1.0 g

Na2MoO4  0.25 g

CuSO4·5H2O   1.0 g

Vitamine solution  1.0 ml

Phosphate supplement   20.0 ml

Distilled water   960.0 ml

pH   8.0

Hutner s salts

次氨基三乙酸  10.0 g

CaCl2·2H2O   3.335 g

MgSO4·7H2O   29.7 g

(NH4)2MoO4   9.25 mg

FeSO4·7H2O   99.0 mg

Metals 44   50.0 ml

蒸馏水   950.0 ml

Metals 44:

Na-EDTA   2.5 g

ZnSO4·7H2O   10.95 g

FeSO4·7H2O   5.0 g


H3PO4   5.0 g

MnSO4·H2O  1.54 g


CuSO4·5H2O   0.392 g

Co(NO3)2·6H2O   0.248 g

Na2B4O7·10H2O   0.177 g

蒸馏水   1.0 L

维生素溶液:  

维生素B12   10.0 mg

生物素   2.0 mg

维生素B1   10.0 mg

D-泛酸钙   5.0 mg

叶酸   2.0 mg

烟酰胺   5.0 mg

维生素B6   10.0 mg

MgSO4·7H2O   9.6 g

CaCl  0.36 g

KCl    2.0 g

NaHCO3  0.06 g

NaBr   0.026 g

Proteose peptone No.3   5.0 g

Yeast extract  10.0 g

Glucose   1.0 g

Distilled water   1.0 L

pH   7.2

0271. CM3营养琼脂

Beef extract   1.0 g


蒸馏水  1.0 L

磷酸盐溶液:

K2HPO4   2.5 g

KH2PO4   2.5 g

蒸馏水   1.0 L

0268. 海水琼脂

Yeast extract   2.0 g

Peptone   5.0 g

NaCl   5.0 g

Agar  15.0 g

Distilled water   1.0 L

pH   7.0


Yeast extract  10.0 g

Malt extract   4.0 g

Glucose   4.0 g

Agar  15.0 g

Sea water   750.0 ml

Distilled water   250.0 ml

pH   7.5

Artificial sea water:

NaCl   28.13 g

KCl  0.77 g

CaCl22H2O   1.6 g

MgCl2 6H2O   4.8 g

NaHCO3  0.11 g

MgSO47H2O    3.5 g

Distilled water   1.0 L

0269. 加盐棒杆菌培养基

Casein peptone (tryptic digest)  10.0 g

Yeast extract   5.0 g

Glucose   5.0 g

NaCl  60.0 g

Agar  15.0 g

Distilled water   1.0 L

pH   7.2-7.4

0270.

NaCl  81.0 g

MgCl2·6H2O   7.0 g

0272. MH培养基

NaCl  60.7 g

MgCl2·6H2O  15.0 g

MgSO4·7H2O   7.4 g

CaCl2  0.27 g

KCl   1.5 g

NaHCO3   0.045 g

NaBr   0.019 g

Proteose peptone No.3   5.0 g

Yeast extract  10.0 g

Glucose   1.0 g

Distilled water   1.0 L

pH   7.2

0273. 10%MH培养基

NaCl  81.0 g

MgCl2   7.0 g

MgSo4   9.6 g

CaCl2  0.36 g

KCl   2.0 g

NaHCO3 (单独灭菌)  0.06 g

NaBr   0.026 g

Proteose peptone No.3    5.0 g

Yeast extract  10.0 g

Glucose   1.0 g

Distilled water   1.0 L

pH   7.5

0274. PYC培养基

Casein peptone (tryptic digest)    5.0 g

Peptone   5.0 g

Yeast extract  10.0 g

Cellulose  10.0 g

滤纸条/滤纸浆   适量

Distilled water   1.0 L

pH   7.5

0275.

Salt solution   1.0 L

Yeast extract   1.0 g

pH 2.0 with 10 N H2SO4

Salt solution:

(NH4)2SO4   1.3 g

KH2PO4  0.28 g

MgSO4·7H2O  0.25 g

CaCl2·2H2O  0.07 g

FeCl3·6H2O  0.02 g

MnCl2·4H2O   1.8 mg

Na2B4O7·10H2O   4.5 mg

ZnSO4·7H2O   0.22 mg

CuCl2·2H2O   0.05 mg

NaMoO4·2H2O   0.03 mg

VOSO4·XH2O   0.03 mg

CoSO4·7H2O   0.01 mg

Distilled water  1.0 L

pH   2.0

0276.

0277. GYM淀粉培养基

Glucose   4.0 g

Yeast extract   4.0 g

Malt extract  10.0 g

CaCO3   2.0 g

Soluble starch  20.0 g

Agar  15.0 g

Distilled water   1.0 L

pH   7.2

0278. BL琼脂

牛肉提取物   3.0 g

示胨  10.0 g

酪蛋白胨   5.0 g

植胨   3.0 g

酵母提取物   5.0 g

葡萄糖   10.0 g

可溶性淀粉   0.5 g

溶液A   10.0 ml

溶液B  5.0 ml

Tween 80   1.0 g

Agar  15.0 g

L-Cys·HCl·H2O   0.5 g

脱纤维马血   50.0 ml

蒸馏水   825 ml

pH   7.2

盐溶液A:

K2HPO4  10.0 g

KH2PO4  10.0 g

蒸馏水   1.0 L

盐溶液B:

MgSO4·7H2O   4.0 g

NaCl   0.2 g

FeSO4·7H2O   0.1 g

MnSO4·XH2O   0.2 g

蒸馏水   100.0 ml

0279. 硅酸盐细菌培养基

Sucrose   5.0 g

Na2HPO4   2.0 g

MgSO4·7H2O   0.5 g

FeCl3   0.005 g

CaCO3   0.1 g

土壤矿物   1.0 g

Agar  15.0 g

Distilled water   1.0 L

pH   7.0-7.2

0280. BHIY琼脂

脑心提取物  37.0 g

酵母提取物  20.0 g

琼脂  15.0 g

蒸馏水   1.0 L

pH   7.4

0281.

可溶性淀粉   5.0 g

KH2PO4   0.5 g

Trace solution   15.0 ml

NiCl2·6H2O   2.0 mg

NaCl  20.0 g

Artificial sea water   250 ml


肝浸取液   150.0 ml

Distilled water   750.0 ml


Yeast extract   0.5 g

Resazurin   1.0 mg

Na2S·9H2O   0.5 g

pH   6.5

Artificial sea water:

NaCl  27.7 g

MgSO4·7H2O   7.0 g

MgCl2·6H2O   5.5 g

KCl  0.65 g

NaBr   0.1 g

H3BO3   30.0 mg

SrCl2·6H2O   15.0 mg

柠檬酸   10.0 mg

KI 0.05   mg

CaCl22·2H2O   2.25 g

Distilled water   1.0 L

pH

维生素溶液:

维生素B12   1.0 mg

生物素   20.0 mg

对氨基苯甲酸   50.0 mg

烟酸   50.0 mg

维生素B1   50.0 mg

D-泛酸钙   50.0 mg

叶酸   20.0 mg

维生素B6   100.0 mg

硫辛素   50.0 mg

维生素B2   5.0 mg

蒸馏水    500.0 ml

还原性溶液:

NaOH (0.2 N)   200.0 ml

Na2S·9H2O   2.5 g


Trace element solution:

次氨基三乙酸   1.5 g

MgSO4·7H2O  3.0 g

MnSO4·2H2O   0.5 g

L-半胱氨酸   2.5 g

微量盐溶液:

次氨基三乙酸   1.5 g

MgSO4·7H2O   3.0 g


NaCl   1.0 g

FeSO4·7H2O   0.1 g

CoSO4·7H2O  0.18 g

CaCl2·2H2O   0.1 g

ZnSO4·7H2O  0.18 g

CuSO4·5H2O  0.01 g

KAI( FeSO4)2·12H2O  0.02 g

H3BO3  0.01 g

Na2MoO4·2H2O  0.01 g

NiCl2·6H2O   0.025 g

Na2SeO3·5H2O   0.3 mg

Distilled water   1.0 L

0282. 嗜热厌氧培养基

MnSO4·2H2O   0.5 g

NaCl   1.0 g

FeSO4·7H2O    0.1 g

Co(NO3)2·6H2O   0.1 g

CaCl2·2H2O   0.1 g

ZnSO4·7H2O   0.1 g

CuSO4·5H2O  0.01 g

KAI (FeSO4)2·12H2O  0.01 g

H3BO3  0.01 g

Na2MoO4·2H2O  0.01 g

Na2SeO3·5H2O   1.0 mg

Distilled water   1.0 L

0283.


KH2PO4   1.5 g

Na2HPO4·12H2O   4.2 g

NH4Cl   0.5 g

MgCl2·6H2O  0.18 g

Yeast extract   2.0 g

Glucose   8.0 g

Vitamin solution  0.5 ml

Wolfe s Modified mineral elixir  5.0 ml

Resazurin (0.1%)  1.0 ml

Reducing solution   40.0 ml

蒸馏水   950 ml

Peptone  10.0 g

Yeast extract   5.0 g

Liver extract  25.0 g

Glucose   3.0 g

Glycerol  15.0 g

NaCl   3.0 g

Distilled water

make up to   1.0 L

Agar  15.0 g

pH   7.0

0284.


Salt base solution   1.0 L

Trace mineral   10.0 ml

Citric acid   5.0 mg

yeast extract  1.0 g

Peptone  5.0 g

Sulfer (Powder)   30.0 g

Na2S·9H2O  0.5 g

Resazurin   1.0 mg

pH   6.5

Salt base solution:

NaCl   13.85 g

MgSO4·7H2O   3.5 g

MgCl2·6H2O  2.75 g

KCl   0.325 g

NaBr  0.05 g

H3BO3  15.0 g

SrCl2·6H2O   7.5 g

KI  0.05 g

CaCl2·2H2O  0.75 g

KH2PO4   0.5 g

(NH4)2Ni(SO4)2·6H2O   2.0 g

Distilled water   1.0 L

Trace mineral: See medium No. 0282

0285. 庖肉培养基

碎牛肉(无脂肪)   500.0 g

蒸馏水   1.0 L

1 N NaOH   25.0 ml

蛋白胨  30.0 g

酵母提取物   5.0 g

K2HPO4   5.0 g

刃天青   1.0 mg

L-半胱氨酸  0.5 g

pH   7.0

0286.

Casein peptone (tryptic digest)  10.0 g

Yeast extract   5.0 g

Na-lactate  10.0 g

Agar  15.0 g

Distilled water   1.0 L

pH   7.0-7.2

0287. TPY medium

Trypticase  10.0 g

Phytone   5.0 g

Yeast extract   2.5 g

Glucose  15.0 g

Tween 80  1.0 ml

K2HPO4   2.0 g

MgCl2·6H2O   0.5 g

CaCl2  0.15 g

ZnSO4·7H2O  0.25 g

FeCl3 trace

L-Cys.HCl   0.5 g

Agar   15.0 g

Distilled water   1.0 L

pH   6.5

0288. EG medium

Lab-lemco meat extract   2.4 g

Proteose peptone No.3  10.0 g

Yeast extract   5.0 g

Na2HPO4   4.0 g

Glucose   1.5 g

Soluble starch   0.5 g

L-Cysteine-HCl·H2O   0.5 g

Horse blood   50.0 ml

Distilled water   1.0 L

pH  7.6-7.8

0289.

Peptone  10.0 g

Yeast extarct   2.0 g

MgSO4·7H2O   0.5 g

Sea water    750 ml

Distilled water   250 ml

Agar  15.0 g

pH    7.2-7.4

0290.

Peptone   10.0 g

Yeast extract   2.0 g

MgSO4·7H2O   1.0 g

NaCl  30.0 g

Distilled water   1.0 L

Agar  15.0 g

pH   7.0

0291.

Glucose   1.0 g

Trypticase   5.0 g

KH2PO4   1.0 g

Na-acetate   4.0 g

Yeast extract   2.0 g

n-Valeric acid  0.1 ml

Resazurin   1.0 mg

Na2CO3  4.0 g

Cysteine-HCl  0.5 g

Distilled water  1.0 L

pH   7.0

Gas atmosphere:100% CO2

0292.

Polypeptone   5.0 g

H3BO3  0.01 g

Na2MoO4·2H2O  0.01 g

Distilled water  1.0 L

0294.

大豆蛋白胨   5.0 g

酵母提取物   5.0 g

葡萄糖  10.0 g

K2HPO4   1.0 g

MgSO4·7H2O   0.2 g


Yeast extract   5.0 g

Soluble starch  10.0 g

K2HPO4   1.0 g

MgSO4·7H2O  0.02 g

NaCl    100.0 g

NaCO3 (单独灭菌)  10.0 g

Agar  15.0 g

Distilled water   1.0 L

pH

0293. 改良MB培养基

NH4Cl   1.0 g

K2HPO4·3H2O   0.3 g

KH2PO4   0.3 g

MgCl2·6H2   0.5 g

NaCl   2.0 g

KCl   0.2 g

CaCl2    0.05 g

Trace element solution    9.0 ml

L-Cysteine.HCl   0.5 g

Yeast extract   1.0 g

Trptone   2.0 g

Soluble starch  10.0 g

Distilled water   1.0 L

pH   7.5

Trace element solution:

次氨基三乙酸   1.5 g

NaCl   5.0 g

Na2CO3(单独灭菌)  10.0 g

琼脂  15.0 g

蒸馏水   1.0 L

0295. TSSY培养基

Trypticase peptone  17.0 g

Phytone peptone   3.0 g

Yeast extract   1.0 g

NaCl  30.0 g

Agar   2.0 g

pH   8.0

0296. 2YT培养基

Tryptone   16.0 g

Yeast extract    10.0 g

NaCl   5.0 g

Agar    15.0 g

Distilled water   1.0 L

pH   7.0

0297. 氨苄青霉素2YT培养基

2TY培养基 (No. 0296)  1.0 L

Ampicillin   100.0 mg

0298. Tet-Amp 2YT agar

2TY培养基 (No. 0296)  1.0 L

Tetracycline   25.0 mg

Ampicillin   100.0 mg

0299. 四环素2YT培养基


MgSO4·7H2O  3.0 g

MnSO4·2H2O  0.5 g

NaCl  1.0 g

FeSO4·7H2O  0.1 g

Co(SO4)2   0.1 g

CaCl2·2H2O  0.1 g

ZnSO4·7H2O  0.1 g

CuSO4·5H2O  0.01 g

KAI( FeSO4)2·12H2O  0.01 g

2TY培养基 (No. 0296)  1.0 L

glucose  10.0 g

Tetracycline   25.0 mg

0300.

KH2PO4   0.2 g

K2HPO4   0.8 g

MgSO4·7H2O   0.2 g

CaCl  0.06 g

FeSO4·7H2O    0.015 g

Fe2(SO4)3·6H2O   0.005 g

Beef extract   1.0 g


Na2MO4·2H2O   0.0025 g

NaCl   0.2 g

CH3COONH4   2.2 g

Sodium citrate   2.0 g

蔗糖  20.0 g

Agar  15.0 g

Distilld water   1.0 L

pH   7.2-7.4

0301. NZCYM培养基

Beef extract   5.0 g

NZ amine (type A)  5.0 g

NaCl   5.0 g

Yeast extract   5.0 g

MgSO4·7H2O   2.0 g

Thymidine   4.0 g

Diaminopimelic acid    10.0 g

Distill water   1.0 L

pH   7.0

0302. 卡那霉素TY培养基

胰蛋白胨     8.0 g

酵母提取物   5.0 g

NaCl   5.0 g

卡那霉素    20.0 mg

琼脂  15.0 g

蒸馏水   1.0 L

pH   7.0

0303.

NaNO3   2.0 g

KH2PO4   1.0 g

MgSO4·7H2O   0.5 g

Yeast extract   2.0 g

Agar  15.0 g

Distilled water   1.0 L

pH   7.0

0304. ML琼脂

Tryptone   10.0 g

NaCl  10.0 g

Yeast extract   5.0 g

Agar  15.0 g

Distilled water   1.0 L

pH   7.0

N-Z amine (type A)  2.0 g

Glucose  10.0 g

Agar  15.0 g

Dist. water   1.0 L

pH   7.3

0306. Organic medium 79

Glucose  10.0 g

Bacto peptone  10.0 g

N-Z amine (type A)  2.0 g

Yeast extract   2.0 g

NaCl   6.0 g

Agar  15.0 g

Distilled water   1.0 L

pH   7.5

0307. 碱性营养琼脂

营养琼脂(No. 0002  1.0 L

用倍半碳酸钠溶液调整至pH9.7.   3.0 g

倍半碳酸钠溶液:

NaHCO3   4.2 g

无水Na2CO3   5.3 g

蒸馏水   100.0 ml

0308. 紫菜培养基

蛋白胨  10.0 g

牛肉提取物   3.0 g

酵母提取物   1.0 g

NaCl  15.0 g

琼脂  15.0 g

紫菜浸取液     1.0 L

pH   7.2-7.4

0309.

Yeast extract   2.0 g

Tryptone   1.0 g

Crotonate  10.0 mMol

Mineral solution  50 ml

Trace metal solution  10 ml

Vitamin solution   5 ml

NaHCO3   3.5 g

Cysteine-sulfide reducing solution  20.0 ml

Resazurin   0.001 g

Distilled water   940.0 ml

pH   7.0-7.5 with NaOH


0305. Bennett s agar

Yeast extract   1.0 g

Gas atmosphere: 80%N2 + 20% CO2

Mineral solution:

KHPO4  10.0 g

MgCl2·6H2O  6.6 g

NaCl   8.0 g

NH4Cl  8.0 g

CaCl2·2H2O     1.0 g

Distilled water  1.0 L

Trace metal solution:

次氨基三乙酸  2.0 g

MnSO4·2H2O  1.0 g

Fe(NH4)2 (SO4)2·6H2O  0.8 g

CoCl2·6H2O  0.2 g

ZnSO4·7H2O  0.2 g

CuCl2·2H2O  0.02 g

NiCl2·2H2O  0.02 g

Na2MoO4·2H2O  0.02 g

Na2SeO4  0.02 g

Na2WO4  0.02 g

Distilled water  1.0 L

pH 6.0 with KOH

维生素溶液:

尼克酸   20.0 mg

维生素B12   20.0 mg

维生素B1   10.0 mg

p-对氨基苯甲酸   10.0 mg

维生素B6   50.0 mg

D-泛酸钙   5.0 mg

蒸馏水  1.0 L

还原性溶液:

L-半胱氨酸  2.5 g

Na2S·9H2O  2.5 g

蒸馏水    100.0 ml

0310. 奶粉、酵母粉培养基

脱脂奶粉   100.0 g

酵母提取物  10.0 g

蒸馏水   1.0 L

自然pH

0311. 察氏酵母培养基(CYA)

NaNO3   3.0 g

KH2PO4   1.0 g

KCl   0.5 g

MgSO4·7H2O   0.5 g

FeSO4·7H2O  0.01 g

酵母膏   5.0 g

蔗糖   30.0 g

琼脂   15.0 g

蒸馏水   1.0 L

0312. YM琼脂

Yeast extract  10.0 g

Peptone  20.0 g

Glucose  20.0 g

Agar  15.0 g

Disrilled water  1.0 L

pH   6.0

0313.

酵母提取物   3.0 g

麦芽提取物   3.0 g

葡萄糖  10.0 g

琼脂   15.0 g

蒸馏水   1.0 L

自然pH

0314.

0315.

5 Be 麦芽汁   1.0 L

蛋白胨   5.0 g

(NH4)2HPO4 1.0 g

CaCO3 6.0 g

琼脂    15.0 g

pH  7.0

0316.

牛肉提取物   5.0 g

蛋白胨  10.0 g

酵母提取物   5.0 g

乳糖   5.0 g

葡萄糖  10.0 g

NaCl   5.0 g

琼脂  15.0 g

蒸馏水   1.0 L

pH

0317.

酵母提取物   3.0 g

蛋白胨   3.0 g

MgSO4·7H2O  0.5 g

CaCl2 0.3 g

蒸馏水   1.0 L

pH  6.8-7.0

0318.

马铃薯    200.0 g

蛋白胨   2.0 g

酵母提取物   5.0 g

蛋白胨  0.5 g


葡萄糖   20.0 g

KH2PO4 3.0 g

MgSO4·7H2O  1.5 g

琼脂  15.0 g

蒸馏水   1.0 L

自然pH

0319.

马铃薯   200.0 g

酵母提取物   5.0 g

葡萄糖  20.0 g

KH2PO4 3.0 g

MgSO4·7H2O  1.5 g

琼脂  15.0 g

蒸馏水   1.0 L

自然pH

0320.

木屑  50.0 g

马铃薯   200.0 g

葡萄糖  20.0 g

KH2PO4 3.0 g

MgSO4·7H2O  1.5 g

琼脂  15.0 g

蒸馏水   1.0 L

自然pH

0321.

马铃薯   300.0 g

蛋白胨   2.0 g

葡萄糖  20.0 g

KH2PO4 3.0 g

MgSO4·7H2O  1.5 g

琼脂  15.0 g

蒸馏水    1.0 L

自然pH

0322.

NH4H2PO4 9.0 g

K2HPO4 3.0 g

NaH2PO4 1.0  g

CaCl2   1.0 mg

MnSO4   1.0 mg

MgSO4·7H2O  1.0 g

FeSO4   1.0 mg

琼脂  15.0 g

蒸馏水   1.0 L

pH   4.5-5.0

0323.

山梨醇   5.0 g

蛋白胨   10.0 g

酵母提取物   2.0 g

NaCl  2.0 g

琼脂  15.0 g

蒸馏水   1.0 L

pH   7.0

0324.

牛肉提取物   3.0 g

蛋白胨  10.0 g

草酸   3.0 g

NaCl  5.0 g

琼脂  15.0 g

蒸馏水   1.0 L

pH   6.4-6.7

0325.

0326.

玉米浆  50.0 g

葡萄糖  20.0 g

(NH4)2SO4   3.0 g

CaCO3  20.0 g

琼脂  15.0 g

蒸馏水   1.0 L

pH   6.8-7.0

0327.

KH2PO4 1.0 g

K2HPO4 1.0 g

MgSO4·7H2O  0.5 g

MnSO4·H2O  0.02 g

FeSO4  0.05 g

尿素   5.0 g

酵母提取物    0.2 g

羧甲基纤维素钠   5.0 g

蒸馏水   1.0 L

pH 6.5

0328.

蛋白胨    10.0 g


ZnSO4   1.0 mg

酵母膏   0.3 g

酵母提取物   2.0 g

水解酪素   2.0 g

NaCl  6.0 g

蔗糖  10.0 g

琼脂  15.0 g

蒸馏水   1.0 L

pH   7.2

0329.

0330.

蛋白胨      5.0 g

牛肉提取物   5.0 g

NaCl  5.0 g

蔗糖  10.0 g

琼脂  15.0 g

蒸馏水   1.0 L

pH   7.0

0331. ISP-4培养基

可溶性淀粉  10.0 g

K2HPO4   1.0 g

MgSO4·7H2O   1.0 g

NaCl   1.0 g

(NH4)2SO4   2.0 g

CaCO3   2.0 g

微量盐溶液    1.0 ml

琼脂  15.0 g

蒸馏水   1.0 L

pH   7.0-7.4

微量盐溶液:

FeSO4·7H2O   0.1 g

MnCl2·4H2O   0.1 g

ZnSO4·7H2O   0.1 g

蒸馏水   100.0 ml

0332.

酵母提取物   1.0 g

葡萄糖   1.0 g

可溶性淀粉   1.0 g

CaCO3    1.0 g

琼脂  15.0 g

蒸馏水  1.0 L

pH   7.0-7.2

0333.

硫酸亚铁·7H2O   0.01 g

氯化钾   0.5 g

琼脂  15.0 g

蒸馏水   1.0 L

pH

0335.

0336.

0337.

豆芽汁   1.0 L

葡萄糖  50.0 g

琼脂  18.0 g

自然pH

0338.

5 Be 糖蜜   1.0 L

尿素   1.0 g

琼脂  15.0 g

pH  5-5.6

0339.

10 Be 糖蜜   1.0 L

尿素   2.0 g

琼脂  15.0 g

pH  5-5.6

0340.

水解液(浓度1.5%)   1.0 L

硫酸铵   3.3 g

磷酸二氢钾  0.63 g

琼脂  15.0 g

pH   5-6

0341.

0342.

0343.

3 Be 麦芽汁   1.0 L

氯化钠  20.0 g

琼脂  20.0 g

pH   3.0-3.2

0344.

3 Be 麦芽汁   1.0 L

氯化钠  30.0 g

琼脂  20.0 g


0334.

可溶性淀粉  60.0 g

硝酸钠   2.0 g

硫酸镁·7H2O  0.5 g

磷酸氢二钾  10.0 g

pH   4-6

0345.

0346.

葡萄糖  30.0 g

磷酸氢二铵   0.7 g

蛋白胨   5.0 g

碳酸钙(灭菌)   2.0 g

天冬素   1.0 g

琼脂  15.0 g

蒸馏水  1.0 L

pH    7.0

0347.

麸皮  50.0 g

豆饼粉  30.0 g

上述成分混合后,加0.2%氢氧化钠溶液1.0 L,煮沸1小时,用盐酸中和到pH7,然后加琼脂2%,溶化后,分装灭菌.

0348.

蛋白胨  5.0 g

牛肉膏   3.0 g

  1.0 L

琼脂  15.0 g

0349.

蛋白胨  10.0 g

磷酸二氢钾   2.0 g

葡萄糖  10.0 g

硫酸镁  0.15 g

琼脂  20.0 g

蒸馏水  1.0 L

pH   5.6

0350.

葡萄糖   1.0 g

牛肉膏   4.0 g

蛋白胨   5.0 g

酵母膏   3.0 g

琼脂  15.0 g

蒸馏水  1.0 L

自然pH

0351.

玉米浆  50.0 g

葡萄糖  20.0 g

硫酸铵   2.0 g

0.1% 二氯化钴溶液  4.0 ml

磷酸氢二钾   8.0 g

琼脂  15.0 g

蒸馏水   1.0 L

pH   6.87.0

碳酸钙  25.0 g

磷酸氢二铵   1.0 g

琼脂  15.0 g

蒸馏水   1.0 L

pH 自然

0353.

葡萄糖   100.0 g

牛肉膏   2.0 g

尿素  15.0 g

硫酸镁  0.5 g

磷酸氢二钾   1.0 g

10%米曲汁   30.0 ml

琼脂  15.0 g

蒸馏水   970.0 ml

pH   7.2

0354.

葡萄糖   200.0 g

硫酸镁      0.5 g

尿素  13.0 g

蛋白胨   5.0 g

磷酸氢二钾   1.0 g

10%米曲汁  5.0 ml

琼脂  15.0 g

蒸馏水   1.0 L

pH   7.2

0355.

牛肉膏   2.0 g

葡萄糖  10.0 g

水解干酪   2.0 g

酵母膏   1.0 g

琼脂  15.0 g

蒸馏水  1.0 L

pH   7.3

0356.

5%麸皮汁   1.0 L

琼脂  15.0 g

pH   7.0

0357.

5%麸皮汁   1.0 L

硫酸亚铁    20.0 mg

琼脂  15.0 g

pH   7.0


0352.

蔗糖  30.0 g

0358.

麸皮   50.0 g

葡萄糖   6.0 g

酵母提取物   1.0 g

琼脂  15.0 g

蒸馏水  1.0 L

pH   7.0

0359.

蔗糖  20.0 g

玉米(或土豆)淀粉  20.0 g

磷酸二氢钾   3.0 g

硫酸锌   0.3 g

硫酸镁   3.0 g

氯化铵   3.0 g

琼脂  15.0 g

蒸馏水  1.0 L

pH   6.8

0360.

磷酸氢二钾   0.4 g

碳酸氢钠   1.5 g

蔗糖    125.0 g

氯化锰    3.0 mg

蛋白胨   4.0 g

琼脂  15.0 g

蒸馏水  1.0 L

pH   7.0

0361.

蛋白胨    6.0 g

硫酸镁  0.2 g

硫酸亚铁   0.005 g

磷酸氢二钾  0.2 g

L-天冬素  0.15 g

甘油   2.0 g

琼脂  15.0 g

蒸馏水   1.0 L

pH   7.2

0362.

酵母提取物  25.0 g

葡萄糖  5.0 g

无水醋酸钠  5.0 g

琼脂  15.0 g

蒸馏水  1.0 L

0363.

酵母提取物  20.0 g

硫酸镁  0.5 g

磷酸氢二钾   0.5 g

葡萄糖  10.0 g

琼脂  15.0 g

蒸馏水  1.0 L

pH   7.8

0364.

磷酸二氢钾  2.0 g

尿素   3.0 g

硫酸镁  1.0 g

玉米浆  1.0 g

硫酸锰  0.02 g

硫酸亚铁  0.2 g

琼脂  15.0 g

蒸馏水  1.0 L

pH 自然(5.5

接种前加无菌液体石蜡数滴

0365.

蛋白胨  8.0 g

葡萄糖   10.0 g

牛肉膏   3.0 g

琼脂  15.0 g

蒸馏水  1.0 L

pH   7.2-7.4

0366.

黄豆饼粉(热榨)    10.0 g

氯化钠   5.0 g

蛋白胨   3.0 g

碳酸钙   2.0 g

甘油  10.0 g

琼脂  15.0 g

蒸馏水     1.0 L

pH   7.07.2

黄豆饼粉加入10倍重量的水,用氢氧化钠调节pH6.8,于8085℃加热搅拌10分钟,过滤,取用滤液。

0367.

氯化钠  5.0 g

牛肉膏  10.0 g

蛋白胨  10.0 g

琼脂  15.0 g

蒸馏水  1.0 L

pH   7.2

0368.

硫酸镁   0.1 g

碳酸钙   4.0 g

葡萄糖  30.0 g

蛋白胨   5.0 g

磷酸二氢钾  0.12 g

磷酸氢二铵  0.23 g

琼脂  15.0 g

蒸馏水  1.0 L

0369.

磷酸氢二钠    1.4 g

蔗糖    100.0 g

蛋白胨   2.5 g

磷酸二氢钾   0.3 g

琼脂  15.0 g

蒸馏水  1.0 L

pH   7.07.2

0370.

蛋白胨  10.0 g

酵母膏   5.0 g

硫酸锰  0.05 g

西红柿汁(V/V)   250.0 ml

葡萄糖  10.0 g

硫酸镁  0.2 g

1%(W/V)半胱氨酸盐酸磷化物   50.0 ml

琼脂  15.0 g

蒸馏水   700.0 ml

pH    4.8

0371.

牛肝(切成小块)   500.0 g

蛋白胨  10.0 g

磷酸氢二钾   1.0 g

可溶性淀粉   5.0 g

磷酸氢二铵   2.5 g

蛋白胨    3.0 g

硫酸镁  0.25 g

琼脂  15.0 g

蒸馏水  1.0 L

pH   7.2

0374.

蔗糖  30.0 g

氯化钾   0.5 g

硫酸亚铁  0.01 g

硝酸钠   2.0 g

磷酸氢二钾   1.0 g

琼脂   15.0 g

蒸馏水   1.0 L

pH   7.2-7.4

0375.

葡萄糖  20.0 g

酵母膏   4.0 g

尿素   2.0 g

琼脂  15.0 g

蒸馏水   1.0 L

pH   4.5

0376.

6 Be 麦芽汁   1.0 L

酵母膏   2.0 g

琼脂  15.0 g

pH   5-5.2

0377.


自来水   1.0 L

新鲜牛肝除去浮皮、脂肪,切碎后取500g1.0 L水中混合,在冰箱中浸泡过夜。1.05kg/cm灭菌10分钟,通过布氏漏斗过滤分出肉渣。

在滤液中加入其它成分,调节pH8.0,用滤纸过滤,并加水使滤液体积恢复到1.0 L

每个试管加牛肝渣至 1.5cm,再加牛肝液,总深度为6.0cm,再加少量碳酸钙。

0372.

蛋白胨   10.0 g

牛肉膏   5.0 g

琼脂  15.0 g

蒸馏水   1.0 L

0373.

葡萄糖   5.0 g

蛋白胨   5.0 g

酵母膏   3.0 g

琼脂  15.0 g

蒸馏水  1.0 L

pH   6.8

0378.

酵母膏  10.0 g

葡萄糖  20.0 g

碳酸钙    20.0 g

琼脂  15.0 g

蒸馏水  1.0 L

0379.

胰蛋白酶  20.0 g

酵母膏  5.0 g

氯化铁    7.0 mg

氯化锰   1.0 mg

硫酸镁   15.0 mg

蒸馏水  1.0 L

灭菌后,用无菌10KOHpH7.3,加果糖,(分别灭菌)0.5%作碳源。(胰蛋白酶可用胰蛋白胨代替)

0380.

胰蛋白胨  10.0 g

酵母浸膏   10.0 g

过滤后的西红柿汁(pH7.0   200.0 ml

琼脂   15.0 g

蒸馏水   800.0 ml

pH   7.2

0381.

葡萄糖  20.0 g

酵母提取物   5.0 g

蒸馏水   1.0 L

0382.

蛋白胨    10.0 g

葡萄糖  20.0 g

琼脂  15.0 g

蒸馏水  1.0 L

pH   5.6

0383.

0384.

葡萄糖   1.0 g

酵母提取物   3.0 g

琼脂  15.0 g

蒸馏水    1.0 L

pH   7.0

0385.

0386.

葡萄糖  10.0 g

酵母膏  10.0 g

K2HPO4 0.5 g

琼脂  15.0 g

蒸馏水  1.0 L

pH   7.2-7.4

0387.

自身糖化米曲汁(浓度为6.5 Be  1.0 L

琼脂  20.0 g

pH   4.5

自身糖化米曲汁(浓度为6 Be   1.0 L

琼脂  20.0 g

pH自然

0389.

蛋白胨  10.0 g

牛肉膏  10.0 g

氯化钠  5.0 g

琼脂  15.0 g

蒸馏水  1.0 L

0390.

20%土豆汁   500.0 ml

20%胡萝卜汁   500.0 ml

琼脂  15.0 g

pH自然

0391.

西红柿汁   200.0 ml

可溶性淀粉   0.5 g

蛋白胨  15.0 g

NaCl   5.0 g

酵母提取物   6.0 g

吐温80   1.0 g

葡萄糖  20.0 g

琼脂  15.0 g

   800.0 ml

pH   7.2

0392.

酵母膏  10.0 g

葡萄糖   3.0 g

蛋白胨   5.0 g

牛肉膏   5.0 g

甘油  15.0 g

20%土豆汁   200.0 ml

碳酸钙  10.0 g

琼脂   15.0 g

  800.0 ml

pH   7.0

乙醇(灭菌后加入)   20.0 ml

0393.

蛋白胨   5.0 g

NaCl   5.0 g

葡萄糖  10.0 g

碳酸钙   0.2 g

琼脂  15.0 g


0388.

蒸馏水   1.0 L

pH   7.2-7.4

0394.

淀粉  20.0 g

天门冬酰胺   0.5 g

葡萄糖    0.5 g

KNO3   1.0 g

K2HPO4   0.5 g

NaCl   0.5 g

FeSO4    0.01 g

琼脂  15.0 g

蒸馏水  1.0 L

pH   7.2-7.4

0395.

红茶叶  10.0 g

白糖   100.0 g

乳酸   20.0 ml

NH4H2PO4   5.0 g

蒸馏水  1.0 L

pH   5.0

0396.

马丁肉汤(培养基0058  1.0 L

脱纤绵羊血   50-100.0 ml

0397.

马丁肉汤(培养基0058  1.0 L

马血清   50-100.0 ml

0398.

马丁肉汤(培养基0058  1.0 L

马血清   200.0 ml

0399.

马丁肉汤(培养基0058  1.0 L

马血清    100.0 ml

0400.

牛心汤   300.0 ml

马血清    200.0 ml

0404.

马丁肉汤(培养基0058  1.0 L

蛋白胨  20.0 g

葡萄糖   2.0 g

Na2HPO4 1.0 g

NaCl  2.0 g

NaHCO3 2.0 g

琼脂   15.0 g

蒸馏水   1.0 L

pH   7.0

0405.

心脏提取液  25.0 g

葡萄糖  10.0 g

蒸馏水   1.0 L

0406.

甘油  15.0 g

L-酪氨酸   0.5 g

L-天冬素   1.0 g

K2HPO4 0.5 g

MgSO4·7H2O  0.5 g

NaCl  0.5 g

FeSO4·7H2O  0.01 g

微量盐溶液   1.0 ml

琼脂  15.0 g

蒸馏水   1.0 L

pH   7.2-7.4

0407.

0408.

麦芽提取物  20.0 g

葡萄糖  20.0 g

蛋白胨   1.0 g

琼脂  15.0 g

蒸馏水  1.0 L

pH   7.0


1%水解乳蛋白Hank氏液   500.0 ml

25%酵母浸出液   20.0 ml

1/80醋酸铊  1.0 ml

青霉素   200.0 U/ml

0401.

胰蛋白胨  20.0 g

NaCl   5.0 g

琼脂  15.0 g

蒸馏水   1.0 L

pH    6.8-7.0

0409.

葡萄糖  10.0 g

酵母提取物  10.0 g

牛肉提取物   4.0 g

蛋白胨   4.0 g

NaCl  2.5 g

琼脂  15.0 g

蒸馏水   1.0 L

pH   7.2-7.4

0410.

葡萄糖  10.0 g

L-天冬素   1.0 g

K2HPO4 1.0 g

甘油     10.0 ml

微量盐溶液   1.0 ml

琼脂  15.0 g

蒸馏水   1.0 L

pH   7.2

0411.

葡萄糖  10.0 g

蛋白胨   5.0 g

牛肉提取物   5.0 g

NaCl  5.0 g

琼脂  15.0 g

蒸馏水   1.0 L

pH   7.2

0412.

可溶性淀粉  10.0 g

K2HPO4 1.0 g

NaCl  1.0 g

(NH4)2SO4   1.0 g

CaCO3 2.0 g

微量盐溶液     1.0 ml

琼脂  15.0 g

蒸馏水   1.0 L

pH   7.2

0413.

玉米浆  10.0 g

葡萄糖  10.0 g

可溶性淀粉  15.0 g


(NH4)2SO4 3.5 g

NaCl  5.0 g

CaCO3   5.0 g

琼脂   15.0 g

蒸馏水   1.0 L

pH   7.0